Deepti Lokanath (Ph.D) Email & Phone Number

Houston, Tx, Us

Houston, Tx, Us

Houston, Texas, Us

Training in SOP , SVP of procedures and protocols. QC training .

Houston, Texas, Us

Involved in Process development , R&D, Assay development Troubleshooting QPCR, ddPCR and NGS projects.Project Manager involved in Data analysis and key POC with clients.Pivote role in writing SVP, SOP Operation and Maintenance of QIAcube connect- An automated high throughput extraction machine.Expertise in viral titer assay.Involved in Process development and Automation of Extraction by qualifying Maxwell 16, automated cell extraction Equipment. Worked on gWIZ cell culture, extraction of plasmid gWIZ using QIAspin mini prep kit and copy number QPCR using 7900.Working as Project manager for several projects which needs study protocol, report generation and report submission.Giving technical guidance and also troubleshooting extraction protocols with blood and various tissuesExpertise in QPCR using QIAagility and ABI 7900, 7500 and Quant studioWorking on assay development, assay validation of QPCR Biodistribution and toxicology projects.Understanding of GLP, cGMP and GCP compliance , Quality Assurance, Quality control and Regulations.

Houston, Texas, Us

cGMP validation assay Project manager working on CAR-T cell assay lot release, RCL test, Residual DNA assay, Vector copy number , Viral titer testing and cell bank testing.Preparing Study plan, Strategy plan for the team, report generation and budgeting along with invoicing the projects.

Houston, Texas, Us

Involved in planning and drafting PCR(QPCR,RT QPCR) Study protocol, designing primers, executing the assay.Train Lab associates if required, complete the projects under given timeline.Attend meetings with clients, present powerpoint presentations on the results and data.Analyse the data and also prepare the reports.Involved in the trouble shooting of ddPCR, QPCR and NGS GLP/cGMP/ research grade projects from various clients.

Mysore, Karnataka, In

Title: Bio-cultural Adaptations: People of India, study on Type 2 diabetes on Gangadikara VokkaligasGangadikara Vokkaligas families were studied to identify the interrelationship between case and control subjects along with anthopometry, psychological and clinical data. The biochemical markers related to Type-2 diabetes and its associated risk factors like CVD was studied.The genetic markers of Type-2 diabetes was studied in case controls using Taqman assay in RT PCR.Fasting plasma glucose, lipid profile, liver function test, kidney function test were analysed using ERBA kits in automated biochemical analyser EM 360.Thyroid profile (T3, T4 and TSH) was analysed using Chemiluminescence technology in Immulite 1000.2 plex assay of insulin and ghrelin using multiplex luminix technology.33 taqman primers of diabetes were studied using Allele discrimination in RT PCR by Taqman assay.

I was involved in the project "People of India: Bio cultural adaptations: Bio cultural risk factor assessment of type 2 diabetes on Gangadikara Vokkaligas" Collection of data regarding their physical activity, clinical history, life style, psychological and socio economic data. I have analysed the BMI , Body fat % measurement using the Bio impedence ..Extraction of DNA samples and also its quantitative check for all of them using spectrophotometer. Thyroid analysis (T3, T4 and TSH) of all the plasma samples using Immulite 1000. 2 plex (Adiponectin and Resistin) analysis of plasma samples in Luminex.I have attended the hands on training on Immulite 1000.I was DNA extraction of second visit samples and quantification of all DNA dilutions. I did the 5plex analysis of plasma samples on luminex. I did the biochemical analysis of second visit serum samples. I am trying to conduct awareness camps in Mysore and surrounding areas by contacting subjects of nearby areas to gather people.The extracted DNA was diluted and RT PCR for 50 cases and 50 control samples were done. The RT PCR was done using Taqman assay. The RT PCR was done for 33 Taqman primers. The alleles were sorted out using taqman genotyper software.

Conducting Theory and Practical classes in Biochemistry and Biotechnology. Theory classes includes oral and powerpoint presentations on Organic and Inorganic Biochemistry, Physiology and Immunolgy , Genetics and Molecular Biology chapters.

I was involved in the "DNA Polymorphism in Contemporary Indian Population and Ancient Skeletal Remains" projectI have extracted DNA from Konda reddy and Koya reddy tribal blood samples.The extracted DNA was quantified using UV spectrophotometerThe PCR was conducted using Mitochondrial primersI have conducted PCR for 15 Tribal samples using M9,M47,M89 primers to check for their snp and to classify their haplotypesthe sequencing of all these samples were done using ABI 3730

Doctor Of Philosophy (Ph.D.), Biochemistry

Master Of Science (M.Sc.), Biochemistry

Bachelor Of Science (B.Sc.), Biochemistry, Biotechnology And Microbiology

Doctor Of Philosophy - Phd, Clinical Research/ Molecular Biology