Denise Butler Email and Phone Number

- Design, author, test, and optimize automated workflows from manual assay development onto liquid handling systems- Analyze current workflows, quantify possible improvements, and develop iterative solutions to continuously improve laboratory procedures and operational performance- Troubleshoot acute and longitudinal issues with assay performance and instrumentation- Develop and document work under design control and quality management systems, policies and procedures- Perform research, development, verification and validation testing- Provides instrument troubleshooting and support- Perform lab work in support of R&D activities - Execute and document test plans and Standard Operating Procedures (SOPs) for automated workflows, including IQ, OQ and PQ and maintenance procedures - Contribute to high quality lab testing development activities in the NGS lab - Identify and evaluate new and emerging technologies in assay development and/or automation - Hamilton Advanced Venus Software and STAR Service Engineer Training certified- Active member of company Culture Task Force focusing on upholding company culture and keeping employees engaged

- Performed a variety of high-complexity laboratory assays according to established procedures, including cfDNA isolation, genomic library preparation and sequencing.- Assisted in the development and optimization of novel laboratory processes.- Independently identified and found solutions to high-complexity problems that could adversely affect the test performance.- Verified instrument performance through inspection, calibration, and maintenance per approved procedures.

- Provided remote NGS data analysis/reporting support to the lab as needed

- Evaluated new tests and performed development projects according to plans and guidelines provided by the Clinical Director and/or Laboratory Supervisor- Successfully completed validation of One Lambda’s AllType Next Generation Sequencing System with Ion Torrent Technology, using the Ion Chef, Ion S5 Sequencer and Type Stream Visual software- Communicated scientific information and progress to the laboratory supervisor and director- Served as an NGS technical resource for the lab- Performed troubleshooting efforts to find solutions to problems with laboratory instruments and procedures, and made necessary corrections- Performed feasibility studies and cost/benefit analysis on new initiatives- Continuously evaluated current instrumentation and procedures to identify quality and efficiency improvements for laboratory methods, technology, and or/instruments- Performed technical functions in the preparation of blood or tissue for molecular diagnostics and special laboratory techniques. Obtained clinical histories and specimen orientations for follow-through testing- Responded to inquires about assays, results, clinical protocols, references ranges or laboratory procedures- Assisted in writing new procedures and helped to maintain a current and carefully edited procedure manual- Interacted with personnel from other departments in a professional and organized fashion- Assisted management in selection of capital equipment and maintaining the budget targets

• Provide guidance and assist sellers/buyers in marketing/purchasing property for the right price under the best terms• Determine clients’ needs and financials abilities in order to propose solutions that suit them• Intermediate negotiation processes, consult clients on market conditions, prices, mortgages, legal requirements and related matters to their benefit and ensure a fair and honest dealing• Perform comparative market analysis to estimate properties’ value• Display and market real property to possible buyers• Prepare necessary paperwork (contracts, leases, deeds, closing statements etc)• Maintain and update listings of available properties• Cooperate with appraisers, escrow companies, lenders and home inspectors• Promote sales through advertisements, open houses and listing services• Remain knowledgeable about real estate markets and best practices

• Planed and performed experiments on a weekly basis under limited supervision• Developed solutions to a variety of technical problems of moderate complexity and scope• Applied scientific principles to achieve project objectives, including the design and execution of experiments and analysis and interpretation of the data utilizing appropriate hardware, software and statistical tools • Was a team player with effective communication skills that presented data to colleagues at weekly technical meetings• Collaborated with Medical, Quality, Regulatory and R&D to draft and execute validation protocols and assist with project planning

• Performed HLA typing on patient/donor/research samples using LabType SSO, SSP and SBT methods • Detected and characterized specific HLA antibodies using solid phase immunoassays • Conducted final review of HLA typing results• Collected and assessed quality control data to evaluate new reagent lots• Maintained quality control of reagents and equipment, i.e. Luminex, thermal cyclers, DNA sequencers• Verified testing orders per SOPs and clinical center protocols

• Supervised the daily tasks of technologists within the intermediate resolution typing section of the molecular lab • Trained and educated new technologists, clinicians and students in molecular biology techniques, including DNA extraction using manual and automated methods, HLA typing using polymerase chain reaction (PCR), sequence specific oligonucleotide (SSO), sequence specific primer (SSP) and sequence based typing (SBT) methods, gel electrophoresis, and interpretation of HLA typing results using HLA Fusion and Assign ATF software• Provided final analysis of all intermediate resolution HLA typing results to ensure accuracy and validity• Verified sequencing data using ATF software• Performed quality control testing for reagents• Documented and maintained records for quality assurance• Monitored inventory and ordered supplies, reagents and equipment as needed• Maintained equipment, i.e. thermal cyclers, DNA isolation machines, spectrophotometer, automated liquid-handling system, and Luminex 100• Developed laboratory procedures for new or improved assays• Assisted in writing and implementing protocols• Effectively communicated procedural updates and changes to staff• Participated in validation studies to introduce new equipment and/or test methodologies to the laboratory• Coordinated data collection and organization for projects with outside institutions, such as disease association and vaccine studies• Performed stat testing on-call for deceased donor cases including, HLA typing, complement-dependent cytotoxicity crossmatches, antibody characterization and strength analysis using solid-phase immunoassays, and then effectively communicated results to lab managers and patient & organ procurement organization (OPO) coordinators

• Performed HLA typing for patients/donors using intermediate and SBT methods• Detected and characterized specific HLA antibodies detrimental to transplantation using solid phase immunoassays, complement-dependent cytotoxicity testing and flow cytometry • Performed stat testing on-call for deceased donor cases including, HLA typing, complement-dependent cytotoxicity crossmatches, flow cytometry, antibody characterization, and then effectively communicated results to lab managers, patient & OPO coordinators• Accurately prepared reagents and operated equipment, i.e. flow cytometer, Luminex 100, Lambda Jet, microscopes, thermal cyclers and DNA sequencer