Brandon Deore Email & Phone Number

• Function lead for cell line development (CLD) projects covering cell transformation, single cell selection and outgrowth, fed batch screening, stability studies, and cell cryopreservation. • Coordinated with manufacturing teams to ensure smooth tech transfer and cGMP bank manufacturing.• Authored SOPS and collaborating with cross-functional teams to gather necessary information and input for the development and refinement of SOPs, protocols, and summary reports related investigations.• Managed project timelines, led teams, and delivered oral and written deliverables. • Deployed python and R based applications to scape and process data • Lead biostatistical analyst leveraging historical data to generate predictive process models• SME for AMBR 250 and 15 for process characterization and clone screening • Extensive experience with alternative culture modes for biologics production including Intensified fed batch and continuous perfusion platforms(ATF/TFF)• Internal development lead for evaluating new equipment to improve process efficiencies• Experience with Pilot scale (50L - 200L), bench scale (3L-15L), and rock motion (10-50L) fermentation systems• Developed BLI based assays for titer evaluation of challenging molecules.

Glassboro, New Jersey, United States

• Uncovered novel components of a mechanosensing complex associated with blood-brain barrier (BBB) formation under fluid shear stress.• Interrogated the effects of SARS-CoV-2 on the BBB and identified RhoA as a key component of disease pathology. • Developed a novel in vitro cell model to study the role of RhoA in multicellular viscoelastic mechanics. • Employed finite element modeling via a commercial program, COMSOL, to study hyper-elastic properties of cell sheets in conjunction with physical testing using a micro-tensiometer • Gained extensive knowledge of the central nervous system (CNS) and neurodegenerative diseases such as Alzheimer’s and Parkinson’s • Developed comprehensive experience in molecular biology techniques including PCR, ELISA, Western Blot, electrophoresis, pull-down, and immunoprecipitation• Engineered and designed different Arduino based peristaltic pump systems for time-dependent and steady fluid flow regimes• Cultured numerous cell types including cerebral microvascular endothelial cells, MSC, dermal fibroblasts, astrocytes, human brain vascular pericytes, coronal smooth muscle cells, lung microvascular endothelial cells, and iPSCs• Differentiated iPS cells into specific phenotypes using defined culture media• Genetically modified multiple cell types using plasmids, lentivirus, adenoviruses, CRISPR, and siRNA• Optimized protein and nucleotide extraction methods for three-dimensional in vitro models• Validated engineered blood vessel functionality using permeability, Trans-Epithelial Electrical Resistance (TEER)and immunofluorescence assays• Studied the effects of fluid flow on ultrasound based vascular disruption for cancer treatment • Characterized bovine blood flow profiles using mPIV in acellular and cell seeded models • Prototyped and tested a novel platform to study hemodynamics in a 3D printed disturbed flow model • Manufactured hypoxic models to study the role of oxygen gradients on angiogenesis and barriergenesis

• Instructed undergraduate students in a wide range of laboratory techniques including mammalian cell culture, finite element modeling, microfluidic device manufacturing and statistical analysis. • Provided feedback and guidance on oral presentations and written reports

• Participated in an annual summer program that hosts high school students from underserved communities• Responsible for overseeing groups of students and leading them through various projects

Doctor Of Philosophy - Phd, Biomedical Engineering, Gpa 4.00/4.00

Bachelor Of Science - Bs, Bioengineering And Biomedical Engineering, Gpa 3.55/4.00