Derek Pappas Email & Phone Number

California, United States

California, United States

Seattle, Washington, Us

San Francisco, Ca, Us

• Consultant for the Genomics Sequencing (GSL) and Computational Genomics Resource (CGRL) Laboratories.• Sequencing Services, Technical Support, Data Analysis, Program Design, and Systems Administration

Coralville, Ia, Us

• Provide pre-sales and post-sales guidance, training, technical support, and assitance for customers using NGS approaches.• Support local sales team for onsite and remote technical customer discussions, presentations, and product trainings including vendor shows and trade shows.• Work closely with marketing and sales to ensure the value of the product line is communicated clearly and effectively to customers.• Maintain working relationships with research and development, product development, manufacturing, and customer support to identify and communicate customer needs for future product development efforts.• Attended scientific conferences and local vendor shows to support territory sales representatives and participated in webinars to highlight the value proposition of our products for specific use cases.

San Francisco, Ca, Us

• Genomics Sequencing (GSL) and Computational Genomics Resource (CGRL) Laboratories.• Sequencing Services, Technical Support, Data Analysis, Program Design, and Systems Administration• Supported Illumina (HiSeq, MiSeq, NextSeq, NovaSeq), PacBio (Sequel, Sequel II), and ONT (MinION) technologies.

Nantes, Pays De La Loire, Fr

• Program design for data analysis including software to assist in NGS primer design, HLA genotyping, simulation modeling of a California based stem cell donor haplobank, and survey design for on-site electronic patient data collection.• Project management and client liaison with academic centers, pharmaceutical and biotech companies to ensure timely delivery of analytical reports and journal publications.

Us

• Construction of a high-resolution HLA genotyping database. Program design for raw multi-platform data parsing, genotype tabulation, and data analysis.• Disease association studies between HLA class II alleles and age-related macular degeneration. • Software design (BIGDAWG) for consistent analysis of immunogenomic data.• Full scale design of pipeline for NGS genotyping of the HLA, KIR and STING genetic systems, including primer design for the highly polymorphic HLA and KIR genetic systems, library preparation, as well as genotyping software design, testing, and implementation.- Amplicon generation for testing oligonucleotide suitability, library preparation, purification and quantitation by quantitative PCR. - Operation of next generation sequencing systems including the Illumina MiSeq and HiSeq 2500/4000 systems for sequence data generation.• Construction and maintenance of local Linux server for software deployment including multi-threaded applications, HLA genotyping software and R Shiny applications. • Excellent proficiency programming in R. Basic proficiency in Visual Basic and Python.

San Francisco, California, Us

• Microarray analysis and biological systems modeling to defining transcriptional signatures promoting neuroprotection in primary cortical neurons and iPSC-derived neurons.-Primary neuronal culture systems, cytotoxicity and survival assays, luminescent and fluorogenic bioassays, and primary cell RNAi/siRNA transfection.• Worked with high resolution, large sample size immunogenetic datasets to measure the effects of sample size on HLA haplotype frequency distributions.

London, Uk, Gb

• Assisted biotechnology and pharmaceutical companies with their clinical development, market strategies and positioning across multiple diverse therapeutic areas.• Monitored competitor business developments, strategies and positioning, publications, markets trends, physician perspective and patient uptake.• Maintained up-to-date knowledge of relevant therapeutic areas, clinical programs, regulatory guidelines, sales and marketing, and life cycle management strategies.• Maintained project timelines including the identification, adjudication and synthesis of project data to support client deliverables, written reports, and power point presentations.• Cultivated relationships with key opinion and thought leaders (KOLs), maintained regular contact with principal investigators, conversed with markets analysts.

• Prepared competitor analysis reports to assist in market strategy for biomedical device groups. Assisted in design of commercialization plan. • Conducted market research interviews, prepared company profiles, and co-wrote market reports.• Researched pharmaceutical companies as potential licensing partners for raw materials company.

San Francisco, California, Us

• Implemented microarray analysis, advanced statistics, and dynamic systems modeling to study type I interferon treatment of CD4+ T cells.• Studied the pathological mechanism of a multiple sclerosis associated IL7R SNP.- Recombinant protein expression, ELISA cytokine receptor assay development.• Evaluated technologies to expedite project completion, including: MACS, qRT-PCR, immuno qRT-PCR, nucleofection, nucleic acid purification, TR/FRET, and various cell based assays.• Experience with complex, multi-dimensional data sets and working knowledge of programs including R, Cytoscape, Pajek, and Excel visual basic.

New Haven, Ct, Us

• Studied the alpha-catenin and F-actin interaction using comparative homology modeling and site-directed mutational analysis. Designed, constructed, and isolated recombinant proteins to measure KD.• Applied protein purification (ligand affinity, IMAC, IEX, SEC) techniques, live cell imaging, confocal microscopy, ultra-centrifugation and co-sedimentation, isothermal titration calorimetry (ITC), and thermal denaturation titration circular dichroism (CD).

New Haven, Ct, Us

• Studied the cellular mechanism of presenilin and its function in the Alzheimer’s disease associated enzymatic complex (gamma-secretase).- Designed, constructed, and isolated presenilin 1 and 2 recombinant proteins.- Extracted and purified integral membrane proteins for a soluble enzymatic assay.• Applied subcloning, site directed mutagenesis, immunoblotting, density gradient separation, protein purification, cell culture, lipid transfection, and live cell imaging.

Boston, Ma, Us

• Studied the human molecular genetics of the metabolic disorder, hereditary fructose intolerance. Applied PCR amplification and manual sequencing to exonic regions of the ALDOB gene.

Ph.D., Cell Biology

Ba, Biochemistry And Molecular Biology