David Feder Email & Phone Number

• As member of the biophysics group within the Biotherapeutics Discovery Department, perform in-depth biophysical analysis of various proteins, including AUC, UPLC/HPLC and DLS. • Fluorescently labeled various proteins and performed AU-FDS experiments in buffer and in serum testing antigen/antibody complex formation and size. • Possess a fundamental understanding of protein structure function relationship.• Drove high priority enzyme kinetic study involving the development of enzymatic assays to test kinetics of a target enzyme and the antibodies that act as inhibitors.• Used GE AKTA system to purify various antibodies using methods in chromatography.• Enzymatically generated Fab fragments and purified them via chromatography, which were used in various aspects of biotherapeutics research, including crystallography and binding studies. • Receive protein samples made by CROs, analyze them and provide feedback as to whether or not they meet standards; help to determine whether proteins will be used for further research.• Work cross-functionally with other teams to transfer findings to wider distribution; present work to supervisor, groups, departmental meetings.• Adhere to safety and quality regulations/guidelines (SOPs) consistently.

Brooklyn, Ny

• Expressed recombinant proteins on fermenter scale• Gained Experience in various methods of protein purification• Proficient in methods of protein analysis and quantification, such as SDS-PAGE and colorimetric assays• Proficient in multiple techniques of protein immobilization. • Experienced in polymer synthesis and performing extensive characterization of polymer products using various methods of chromatography and spectroscopy• Conducted in-depth studies comparing activity, selectivity and thermostability of novel and commercially available enzyme catalysts for chemical transformations• Successfully synthesized hyperbranched polyester polyols (HBPs) using various enzyme catalysts and conducted comprehensive architectural and chemical comparison of products• Collaborated through our NSF sponsored industrial/university center with major companies in the chemical, pharma and biotech world

• Guided Graduate students in laboratory techniques and research methodologies

Phd, Biomedical Engineering (Materials)

Dissertation: Humicola insolens cutinase; a novel catalyst for polymer synthesis reactions Thesis adviser: Professor Richard A. Gross.

Ms, Systems Engineering