David Gagne Email & Phone Number

Billerica, Massachusetts, United States

Wlatham, Ma

• Developed techniques and procedures, write protocols, and execute studies both in the Vivarium and at the lab bench.• Trained team members and execute studies utilizing Automated In Vivo Sampling Systems, Electrolyte and Blood Gas Analyzers, EchoMRI Body Composition Analyzer, and WES/JESS for “blot free” Western Blots.• Ran POC studies in iPSC derived Human Neurons and various cell lines • Managed sample collections from ongoing studies and final takedowns (necropsy). • Developed sample preparation techniques to detect a variety of proteins from various tissues and brain regions. • Measured targeted proteins or “biomarkers” by Western Blot utilizing multiple protocols including JESS a “blot free” WB and more traditional gels with and the “iBlot” transfer system. • Processed tissue samples to isolate RNA and cDNA and run qPCR.• Administered drugs to rats via subcutaneous, intraperitoneal, intravenous, and oral gavage. • Ran rat Pharmacokinetic assays collecting blood samples via sublingual, submandibular. • Collected and analyzed data, generate statistics, construct graphs, and write reports.• Operated relative equipment such as the Odyssey Western Blot Scanner, Incucyte live cell imager, Opera Phenix Plus confocal imager, InvivO 300 Hypoxia Workstation, Nanostring nCounter Pro gene expression Analysis System, KingFisher Apex automated RNA purification system.• Active member of Talent, Engagement, and Innovation (TEI) “Rotations” Team developed to locate and offer internal on the job training opportunities for R&D employees in departments within Alkermes• EHS Safety leader CPR and AED trained.

Waltham, Ma

Waltham, Ma

• Scheduled and coordinated all in-house animal studies. • Wrote, reviewed, and served as Principal Investigator (PI) on all IACUC protocols.• Conducted pharmacokinetic, drug tolerance, and chronic dosing studies.• Prepared compound formulations, administered via subcutaneous, intraperitoneal, intravenous, oral gavage, and ICV.• Collected blood via tail, retro orbital, cardiac puncture.• Ran Insulin ELISA’s, Free Fatty Acid assay’s, Clinical Chemistry for various markers.• Maintained Vivarium; mouse orders, sentinel program, supplies, tissue bank• Presented analyzed data, generated statistics, constructed graphs and Power Point presentations.• Served as point person for multiple offsite collaborations and contractors. • Assisted in locating, evaluating, and securing Vivarium space. • Designated and equipped Animal Holding and Procedure rooms.• Managed team of 6 Research Assistants • Mouse Models of metabolic disorders, Cisplatin induced Kidney Injury, LPS induced TNFα release.

• Neurodegenerative Mouse Models R6/2 HD, Quinolinic Acid, 3-NP Striatal lesion HD.• Administered drugs to mice via subcutaneous, intraperitoneal, intravenous, oral gavage, and ICV.• Performed Stereotaxic Surgery; Intracerebroventricular (ICV) and Intrastriatal injections.• Conducted RotaRod behavioral studies, PK/PD, tolerance and chronic dosing studies.• Prepared compound formulations for ongoing studies.• Maintained Vivarium; mouse orders, Sentinel program, supplies, lab supplies and equipment.• Prepared mouse tissues and performed BCA Protein and HDAC activity assays. • Wrote IACUC protocols.• Assisted in locating, evaluating and securing Vivarium space. • Designated and equipped Animal Holding and Procedure rooms.• Trained and directed three Research Assistants on lab bench techniques and surgical procedures.

• Neurodegenerative Mouse Models research SOD1 ALS mouse model.• Performed Chronic dosing studies monitoring disease progression via multiple routes of administration.• Performed Stereotaxic Surgery; placement of ICV cannula for continuous delivery of drug via mini-pump.• Collected mouse tissues and blood plasma for outsourcing; spinal cord, whole brain.• Performed ELISA and Western Blots.• Served as IACUC representative.• Managed shipping and receiving of biological waste and cataloged MSD Sheets for drug and chemical library.• Assisted with moving Company to new location.• Trained staff on communication of company mission statements to the public.

• Conducted experiments and surgeries on balb/c and hrs/jhr (hairless) Mice, and Sprague Dawley Rats.• Administered drugs to mice and rats and collected blood via tail vein and cardiac stick.• Assisted in Rat Stroke Model experiments; pre and post-surgery procedures.• Maintained Vivarium; ordering animals and material, animal husbandry. • Trained research professionals from BU and UMASS in surgical procedures for subcutaneous ALZET pump implantation.• Maintained several cell lines and performed cell membrane permeability screening and fluorescent labeling of cells.• ELISA, BCA Protein Assay, TCA Precipitation of radio-labeled Ab.• Operated Microplate reader, Spectrometer, and Scintillation counter.

Engineering

Activities and Societies: Delta Kappa Phi

As Science, Biotechnology