Di Chu, Ph.D. Email & Phone Number

Boston, Massachusetts, United States

Boston, Massachusetts, United States

Boston, Massachusetts, United States

• Responsible for development and implementation of in-vitro and ex-vivo phenotypic assays to assess function of smooth muscle cells. Perform analyses of signaling pathways mediated by extracellular nucleotides.
• Support identification and validation of potential therapeutic targets and biomarkers analysis. Maintain cell culture and perform primary cell isolation.
• Contribute to the planning, design and execution of experiments and data analysis.
• Present data at internal and external meetings.
• Diligently maintain ELN records and contribute to writing study reports, regulatory submissions, patent applications and publications in peer-reviewed journals, as appropriate.

Tarrytown, NY

• Hands-on experience in the area of protein characterization with focus on liquid chromatography, specifically mixed mode chromatography (MMC).
• Develop MMC method to optimize buffer and column conditions to have enough separation in retention times between bispecific antibodies and mAb impurities to serve as an alternative for existing method.
• Present the project to the entire Protein Biochemistry group at the end of the internship.

Madison, Wisconsin Area

• Research epigenetics of cell fate change using mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)
• Perform molecular biology and biochemistry experiments in the area of somatic cell reprogramming
• Be responsible for ordering supplies, maintaining lab records and inventories, and keeping lab compliant with various safety and other protocols

Skokie, IL

• Lead projects characterizing gene expression in chronic rejection of human aortic smooth muscle cells, kidney and aorta tissues in rat and mouse models (recover RNA from samples, prepare cDNA for qRT-PCR from RNA.
• Successfully optimize and establishe the protocol for RNA recovery from mouse aorta samples.
• Conducte experiments to evaluate relative importance of selected genes in graft rejection and immune system, their drugability, and commercial potential as drug target targets for immunosuppression.
• Independently analyze data using statistic tools and improved upon current protocols.
• Successfully excute and characterize different strains of mouse embryonic stem cell culture and mouse feeder cell culture.
• Develope a new drug-testing model using mouse embryonic stem cells.

Edmonton, Canada Area

• Studied physiologically functional molecules, focusing on the voltage-gated potassium channels.
• Worked on a project studying the key amino acids required for the function of mouse voltage-gated Kv1.2 potassium channel.
• Reconstructed the channel by introducing mutations in its protein sequence and validated by sequencing.
• Expressed the reconstructed channel in Xenopus frog eggs to study its function.
• Demonstrated significance of selected amino acids on functional ability of voltage-gated potassium channel by two-electrode voltage clamp.

Business Certificate For Scientists And Engineers

A non-credit summer program provided by Isenberg School of Management to graduate students in STEM, which aims to supplement traditional.

Doctor Of Philosophy - Phd, Cell/Cellular And Molecular Biology

• Study structural biology of glycoproteins in lysosome. • Investigate potential drug candidates for sialidosis, a rare lysosomal storage.

Master Of Science (M.S.), Biotechnology

Courses taken: Bioprocess Engineering I: Kinetics, Energetics and Bioreactor Design, Bioprocess Engineering II: Properties and Separation.

Bs With Honors, Molecular Genetics

Courses taken: Infection and Immunology, Molecular Genetics and Related Techniques, Microbiology, Cell Biology, Biochemistry