I am responsible for the performance and documentation of Preventive Maintenance, Qualification & Repairs on the equipment in our clients' GMP-regulated pharmaceutical laboratories. Alongside performing maintenance activities and calibrations, I provide lab support and scientific services to meet the customer's needs.Preventive Maintenance:-Agilent HPLC-Agilent GC-Waters Alliance HPLC-Waters Acquity UPLC-Thermo Dionex IC-Thermo Ultimate HPLC-Total Organic Carbon (TOC) Analyzer: Sievers 900 Laboratory (GE)-Water Purifier System: Millipore MilliQ A10 Integral 10-Pipette Calibrator System: Artel PCS3-Spectrophotometers: Beckman DU800, PerkinElmer Lambda25/35/45-Platereaders: Molecular Devices Vmax, Spectramax 384+, Soectramax M3-Atomic Absorption: PerkinElmer AAnalyst 400 -Heat Block: Grant QBH2-Plate Washer: BioTek-DNA Analyzer: Molecular Devices Threshold-Mettler Toledo Balances-Empower 2 & 3 chromatography software-Chromeleon chromatography software

-Managed an Instrument Maintenance Program in client’s FDA-regulated cGMP pharmaceutical Laboratory-Coordinated PM + Metrology events interdepartmentally between QC/QA/Metrology/Lab Equipment Qualification-Active participant in sitewide effort to meet critical due dates and avoid Deviations under Consent Decree conditions

BSR is a contract research organization located in Cambridge, MA that specializes in providing the service of biological assays for its clients in a GxP compliant, quality controlled environment. Laboratory data is sent to the FDA on behalf of clients in the biotech industry. My responsibilities included, but were not limited to, the following:-Human Blood Processing and Separation Techniques. -Flow Cytometry staining and Analysis assays such as CD34+ and p-Selectin via C6 Accuri Cytometer. -Mammalian Cell & Tissue Culture (maintenance of cell lines, cell staining). -ELISA Assays (growth factors such as PDGF, VEGF, TGF, SDF). -Spectrophotometric Assays (SpectraMax spectrophotometer) -Sterility Testing (BacT Alert3D instrument)-QA/QC Temperature Monitoring of Refrigerators & Freezers, Maintenance and Verification of C6 Accuri Cytometer, Maintenance and Verification of new equipment (ex: NOVA Prime ABG Analyzer), Verification of Consolidated Steromaster MKII autoclave, Verification of Beckman Coulter AC Tdiff2 Hematology Analyzer & Medonic CA620 Hematology Analyzer-Writing/Developing protocols for Quality Systems, Metrology, and Technical Methods.I developed and performed a Transparency Test experiment for a client in compliance with ISO-3826-1. I also developed an assay for the measurement of collagenase activity in digested human lipoaspirate.I've obtained the following Biosafety Training Certificates of Completion via Stericycle: Hazard Communication Awareness, DOT Hazardous Materials, Biohazardous Waste, Bloodborne Pathogens

Worked in the laboratory of Dr. Cintia Hongay on an ongoing research study regarding the role of the IME4 gene in the Notch signaling pathway. Drosophila fruit flies and Zebrafish were the model organisms used in the research. With the help of Dr. Kenneth Wallace and his laboratory, the function of the IME4 gene is being explored.