We produce over 3 millions citrus roots stock liners in tissue culture.

Assist in managing and maintaining experiments with focus on propagation and hardening of citrus rootstocks from cuttings and micropropagation in phyto-sanitary greenhouse. Duties include organization and scheduling of experiments; acquisition, recording, maintaining and manipulation of data; and maintenance of tissue cultures and cuttings during hardening-off experiments and rooting experiments.

Research on nursery production of citrus trees in greenhouse environment with emphasis in development of best irrigation methods. Duties included all tasks related with tree production from seeding rootstocks, potting, pruning, pest and disease scouting, etc. and setup and maintenance of experiments.

Played a major role to organizing numerous parameter-optimization experiments of Agrobacterium-mediated transformation, transform cell lines, regenerate genetically modified plants, monitor progress, analyze data and prepare presentation-ready summary materials. Testing of numerous experimental vectors using transgenic tobacco. Played integral part in developing a reliable and versatile method for high throughput functional analysis of resistance genes. Managerial and training of personnel functions included supervising several technicians and student apprentices in the technology and protocols of the laboratory. Proficient in E. coli transformation, agrobacterium transformation, and DNA cloning using the gateway cloning system. Have utilized PCR to isolate DNA fragments of interest. Proficient using DNA extraction methods. Familiar with tissue culture techniques.

Emphasis in whole plant and population water relations, including modeling woody plant water use as a function of plant size and evapotranspiration, developing irrigation coefficients, and weighing lysimeter data analysis.

Extensive experience with amino acid and carbohydrate analysis in HPLC; designed and conducted experiments with large-scale data collection; worked with various software for root growth analysis, and soil moisture probes; designed and developed rhizotron and automatic roots separator.

My duties included monitoring irrigation, application of plant growth regulators, space allocation, planting, preparing growth media, fertilization, insect and disease control, and establishing and monitoring plant growth environments.

Experience with ethylene and 1 MCP analysis in gas chromatography; designed and conducted experiments with large-scale data collection in physiology postharvest.

Worked with protein determination, isolation of mitochondria and peroxisomes, tissue respiration rates and other plant biochemistry analyses.

Sample preparation for ICP analysis (Inductively Coupled Plasma spectrometry), data processing, organic matter determination and particle size distribution of soils. Worked with analytical and experimental techniques for measurements of soil characteristics such as TKN, phosphorus, and ammonium.

Conducted experiments with vermicompostage, analyzed morphophysiology of worms exposed to heavy metals, experienced with field research, wrote scientific reports.