Investigate mechanism of non-small cell lung cancer (NSCLC) survival and progression in brain and cerebrospinal fluidUtilize cells free and single cell RNA sequencing to study tumor-glia cells cross-talk in the brainMethods:Extraction of live cells from fresh brain tissueIsolation of high-quality RNA from fresh human-derived specimenSingle-cell library preparation and RNAseqDevelop and utilize brain-metastases in-vivo models

Investigated SAR of complex macromolecules for efficient in vitro delivery and activity, evaluated cellular internalization, trafficking, toxicity and activity in murine and human cancer cells.Developed and executed the in vivo pipeline to profile polymer-siRNA complexes in PK, PD, tolerability and efficacy studies. Methods:Cell Culture - Cancer cell growth and purification from tumor tissue; Endothelial cells growth and purification from human umbilical vein.Angiogenesis Assays In Vitro - Migration; Proliferation; Capillary-like tube formation; Invasion; Adhesion and Wound Healing.Ex-vivo Toxicology Assays - Red blood cells lysis; Complement activation; Cytokines secretion. Cell-based assays - Cell proliferation assays; Flow cytometry analysis; In vitro silencing assays using different type of cell lines (cancer and primary). In vivo assays - Injection techniques: Intraperitoneal; Intravenous; Subcutaneous. Antitumor efficacy evaluation of chemotherapeutics/oligonucleotides vs. polymer-drug/oligonucleotides conjugates. Toxicology evaluations - Non-invasive imaging using fluorescence and bioluminescence imaging systems. Biodistribution studies. Pharmacokinetic evaluations.

Basic and advanced pharmacology courses at leading nursing and medical schools in Israel