Dino Leone

Dino Leone Email and Phone Number

Neurobiology and Bioinformatics Consultant @
Dino Leone's Location
San Carlos, California, United States, United States
Dino Leone's Contact Details

Dino Leone personal email

n/a

Dino Leone phone numbers

About Dino Leone

Dino Leone is a Neurobiology and Bioinformatics Consultant at Self-employed. He possess expertise in molecular biology, stem cells, fluorescence microscopy, cell culture, immunohistochemistry and 25 more skills.

Dino Leone's Current Company Details
Self-employed

Self-Employed

Neurobiology and Bioinformatics Consultant
Dino Leone Work Experience Details
  • Self-Employed
    Neurobiology And Bioinformatics Consultant
    Self-Employed Mar 2024 - Present
    At the intersection of classical neurobiology and modern next-gen sequencing, harnessing 20+ years of experience in cell and molecular biologyProviding custom analyses for bulk and single cell RNAseq datasets, including full processing of raw sequencing data.Specializing in toplevel analyses to answer systems biology questions such as cell-cell signaling and interactionsStrong experience in analysis pipeline construction; integration with target development pipelines and mode of action elucidationExperiment design and protocol optimization for FACS-based single cell RNAseq/nuc-seq workflows.
  • Alkahest, Inc.
    Director, Transcriptomics
    Alkahest, Inc. Mar 2023 - Dec 2023
    San Carlos, California, Us
    Leading internal group, focusing on bulk and single cell RNA-seq, ATAC-seq and additional molecular biology techniques to support internal target identification and validation.
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  • Alkahest, Inc.
    Associate Director
    Alkahest, Inc. Jan 2020 - Feb 2023
    San Carlos, California, Us
    In charge of our transcriptomics unit to elucidate molecular mechanisms of aging cellular responses to plasma fractions treatments. Also leading our in vitro group developing screening assays for characterization of plasma fractions' effects on cells.
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  • Alkahest, Inc.
    Senior Scientist
    Alkahest, Inc. May 2019 - Jan 2020
    San Carlos, California, Us
    View
  • Alkahest, Inc.
    Scientist Iii
    Alkahest, Inc. Feb 2018 - Apr 2019
    San Carlos, California, Us
    We're employing state-of-the-art single cell RNA-seq technologies to investigate the molecular and cellular mechanisms of aging. I've setup both the lab and bioinformatics infrastructure (custom data analysis platform) to process scRNA-seq.
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  • Coda Biotherapeutics
    Senior Scientist
    Coda Biotherapeutics Oct 2017 - Jan 2018
  • Circuit Therapeutics
    Scientist Iii
    Circuit Therapeutics Oct 2016 - Oct 2017
    In charge of Next-Generation-Sequencing efforts at Circuit Therapeutics. Successfully established single cell RNA-seq protocols using both a Fluidigm C1 and manual protocols such as Clontech SMARTer.
  • Circuit Therapeutics
    Scientist Ii
    Circuit Therapeutics Jul 2015 - Sep 2016
    In charge of implementing Next-gen sequencing. Successfully set up single cell RNA-seq using Fluidigm C1 and manual protocols and subsequent library generation.Established quality control pipeline including picogreen assays and custom C1 protocols for optimized single cell RNA-seq.Successfully designed and setup bioinformatics pipeline for single-cell RNA-seq data analysis using tools such as cutadapt, STAR, HiSat and Cufflinks. Data handling using Python and visualization using matplotlib and Bokeh.
  • Stanford University
    Research Scientist
    Stanford University Jul 2008 - Jul 2015
    Stanford, Ca, Us
    In charge of several research projects while providing training and guidance to undergraduates, graduate students and postdoctoral fellows in the lab.• Investigating differentiation and fate determination of pyramidal neurons in the cerebral cortex. I have identified two transcriptional adaptor proteins, Ldb1 and Ldb2, which are required by corticospinal motor neurons for sending their axonal projections into the spinal cord. Loss of the two proteins leads to a differentiation defect and a loss of the corticospinal tract.Results have been published in 2016 paper “Compensatory actions of Ldb adaptor proteins during corticospinal motor neuron differentiation.”• Established and optimized a method for FACS sorting of live, transiently fluorescent-labeled primary neurons to gain pure neuron populations for microarray expression analysis.• Wrote manuscripts and successfully funded grant applications.• Coordinated projects with collaborators and core facilities.
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  • Stanford University
    Postdoctoral Fellow
    Stanford University Jul 2003 - Jul 2008
    Stanford, Ca, Us
    • Investigated the regulation of neural progenitor proliferation and differentiation by Rho GTPases (Cdc42 and Rac1)-dependent mechanisms in neural stem cells. Demonstrated that Rac1 is required for proliferation of stem cells and intermediate progenitors. Rac1 mutants displayed depletion of cycling progenitors and ectopic expression of cyclin D1, normally expressed by stem cells and intermediate progenitors in the intermediate zone. Results have been published in 2010 paper “The Rho GTPase Rac1 is required for Proliferation and Survival of Progenitors in the Developing Forebrain.”• Established protocols and procedures for in utero microinjection of recombinant adenoviruses.• Developed a client/server database, which manages mouse colonies, including matings, experiments and protocols; subsequently commercialized and sold database to several labs.
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  • Eth, Swiss Federal Institute Of Technology, Zurich
    Ph.D.
    Eth, Swiss Federal Institute Of Technology, Zurich May 1999 - Jun 2003
    • Generated and characterized several transgenic mouse lines expressing a Tamoxifen-inducible variant of the Cre recombinase in oligodendrocytes and Schwann cells (PLP-CreERT2 and P0Cx32-CreERT2).• Investigated the roles of beta1 integrins in neural stem cells and oligodendrocytes and generated a recombinant adenovirus which expresses the Cre recombinase. Loss of beta1 integrins led to a reduction in stem cell proliferation and increased cell death, suggesting that the interaction between stem cells and their extracellular matrix niche is crucial for survival of neural stem cells. I discovered that high levels of beta1 integrin expression in neural stem cells correlated with the capacity for self-renewal, and conversely, blocking integrin function reduced the number of neurospheres, suggesting that integrin function is required for neural stem cell maintenance.• Assessed the roles of beta1 integrins during myelination and differentiation of oligodendrocytes; employed both an in-vitro approach (using adenovirus) and an in-vivo approach. Revealed that beta1 integrins are required for survival of premyelinating oligodendrocytes, but are dispensable during myelination.

Dino Leone Skills

Molecular Biology Stem Cells Fluorescence Microscopy Cell Culture Immunohistochemistry In Vivo Confocal Microscopy Molecular Cloning Cell Biology Qpcr Genetics Microarray Microarray Analysis Immunofluorescence Rnaseq Molecular Genetics Next Gen Sequencing Shell Scripting Database Design Rna Seq Data Analysis Data Visualization Using Matplotlib And Bokeh Single Cell Rna Seq Ngs Library Generation Rna Seq Data Analysis Using Python Epigenetics Oligodendrocyte Differentiation Adenovirus Linux System Administration Unix Shell Scripting Python

Dino Leone Education Details

  • Swiss Federal Institute Of Technology (Eth), Zurich, Switzerland.
    Swiss Federal Institute Of Technology (Eth), Zurich, Switzerland.
    Neuroscience
  • University Of Zurich, Switzerland
    University Of Zurich, Switzerland
    Biochemistry And Immunology
  • University Of Zurich, Switzerland
    University Of Zurich, Switzerland
    Biochemistry

Frequently Asked Questions about Dino Leone

What company does Dino Leone work for?

Dino Leone works for Self-Employed

What is Dino Leone's role at the current company?

Dino Leone's current role is Neurobiology and Bioinformatics Consultant.

What is Dino Leone's email address?

Dino Leone's email address is dl****@****ttx.com

What is Dino Leone's direct phone number?

Dino Leone's direct phone number is +165032*****

What schools did Dino Leone attend?

Dino Leone attended Swiss Federal Institute Of Technology (Eth), Zurich, Switzerland., University Of Zurich, Switzerland, University Of Zurich, Switzerland.

What skills is Dino Leone known for?

Dino Leone has skills like Molecular Biology, Stem Cells, Fluorescence Microscopy, Cell Culture, Immunohistochemistry, In Vivo, Confocal Microscopy, Molecular Cloning, Cell Biology, Qpcr, Genetics, Microarray.

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