Main Project: Dissecting the molecular pathways that regulate Foxp3-mediated gene expression and regulatory T (Treg) cell functionTherapeutic Implication: Results generated will provide novel insights into Treg biology and help develop better therapeutics for autoimmune diseases and cancerMajor Scientific Accomplishments:•Generated an interaction map of Foxp3-associated factors involved in Treg cell-specific transcriptome through application of a mass spectrometry based proteomic approach•Investigated the role of the RUNX transcription factors in Treg cells. Demonstrated that the Runx-Cbfβ transcription complexes serve as an essential determinant of the Treg lineage stability•Identified Stat3 as a key interacting partner of Foxp3 and demonstrated its role in Treg cell mediated control of TH17 dependent inflammation in the gut•Investigated the molecular mechanism of divergent susceptibility to diabetes and arthritis resulting from a common insertional mutant of Foxp3