Divya Murthy, Phd Email & Phone Number

Providence, Ri, Us

Providence, Ri, Us

Providence, Ri

Omaha, Nebraska, United States

Tumor microenvironment-mediated metabolic reprogramming in pancreatic cancer. I work on understanding the role of metabolites on the survival and aggressiveness of pancreatic cancer. My work deals with:• Identification of metabolites by Liquid Chromatography based metabolomics (LC-MS/MS).• Identification of genes that potentially promote an aggressive cancer phenotype in pancreatic cancer using Chromatin Immunoprecipitation and RNA-Seq• Proteome analysis using mass spectrometry.• Developing CRISPR-Cas9-based knockout models.Technical Skill set:• Phospho-proteomic studies in pancreatic cancer cells using Reverse Phase Protein Arrays• Tumor cell culture and in-vitro drug testing using spectroscopic and fluorescence microplate assay• Biomarker assay using immunohistochemistry, western blot, and quantitative real-time PCR assays.• LC-MS/MS-based metabolomic studies• Development of tumor organoid-based model system.• GSEA of biological datasets to determine biologically and statistically significant set of genes.https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-23-0334/730024/Vitamin-B6-competition-in-the-tumorhttps://www.mdpi.com/1422-0067/24/8/7304https://www.sciencedirect.com/science/article/pii/S2213231722000738?via%3Dihubhttps://www.nature.com/articles/s41388-021-02132-6https://www.gastrojournal.org/article/S0016-5085(21)03159-0/fulltext?referrer=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2Fhttps://www.sciencedirect.com/science/article/abs/pii/S0304383520303815?via%3Dihubhttps://rupress.org/jem/article/217/7/e20190745/151806/SIRT1-NOX4-signaling-axis-regulates-cancerhttps://www.frontiersin.org/articles/10.3389/fphys.2018.00335/fullhttps://link.springer.com/protocol/10.1007%2F978-1-4939-7665-2_8https://www.sciencedirect.com/science/article/pii/S1535610817302544?via%3Dihubhttps://www.sciencedirect.com/science/article/pii/S0304383517302902?via%3Dihubhttps://www.bioscience.org/2017/v22/af/4543/fulltext.htm

New Delhi, India

Understanding the melanosome and mitochondria dynamics during pigmentation.• Delineated the role of autophagic machinery in melanosome biogenesis and degradation. • Characterized a non-canonical role of autophagic protein MAP1LC3B in mediating movement of melanosomes during melanogenesis. • Identified the motor protein (kinesin) that mediate translocation of melanosomes in melanocytes. • Explored the melanosome-mitochondria crosstalk during melanogenesis using a compendium of tools and techniques including mass spectrometry, mammalian cell culture, and extensive confocal based microscopy.• Utilized organotypic culture derived from human subjects for isolation of melanocytes to validate cell line-based findings.Technical Skill set:• Organelle isolation and its biochemical characterization• In vitro biochemical assays• Molecular biology techniques: Cloning of mammalian genes, site directed mutagenesis, recombinant protein expression in E.coli, protein purification and enzyme activity studies, protein-protein interaction studies by co-immunoprecipitation and protein pull down assays, DNA and RNA isolation and purification.• FACS-based studies• Extensive training in confocal based microscopic analyses of subcellular organelles and structures; and live cell imaging to understand organelle dynamics and kinetics.https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001634https://www.tandfonline.com/doi/full/10.1080/15548627.2017.1327509https://www.sciencedirect.com/science/article/pii/S0006349515009923?via%3Dihubhttps://www.sciencedirect.com/science/article/pii/S0958166913000232?via%3Dihub

Institute Of Biochemistry Ii, Frankfurt Am Main, Germany

Elucidating the role of PLEKHM2 in melanosome trafficking on cytoskeletal tracks in melanocytes.• Collaborated on a research project to elucidate the role of the adaptor protein, PLEKHM2 (SKIP), in mediating movement of melanosomes within melanocyte.• Performed exogenous overexpression and confocal-based experiments to study subcellular localization of PLEKHM2 on melanosomes.• Extensively employed microscopy to analyze subcellular organelles and structures, and live cell imaging to understand organelle dynamics and kinetics.https://www.biorxiv.org/content/10.1101/2021.03.11.434917v1

Vadodara, India

Cytokine expression profiling in Caco-2 and RAW 264.7 cell lines exposed to Lactobacilli isolates to evaluate their probiotic effect.• The project aimed at understanding the modulation in the cytokine profiles of pro- and anti-inflammatory cytokines in Caco-2 colon carcinoma cell lines and RAW 264.7 macrophage cell lines stimulated with various Lactobacilli isolates. • Isolated various Lactobacilli strain from different sources, stimulation of Caco-2 and RAW 264.7 cell lines with the Lactobacilli isolates.• Performed bacterial adhesion assays and semi-quantitative PCR-based cytokine profiling.The project yielded interesting findings. I observed significant immunomodulation on stimulation with various Lactobacilli isolates implicating them as potential probiotic candidates.Technical expertise attained: • Mammalian cell culture, semi-quantitative PCR, bacterial adhesion assays.

Doctor Of Philosophy - Phd, Biotechnology

Master'S Degree, Biotechnology

Bachelor'S Degree, Biochemistry

Higher Secondary Education, Cbse