Dorothy Keine, Phd Email and Phone Number

3Prime creates accurate and well-written scientific and medical deliverables customized to meet your needs. Whether you’re communicating with experts, sales teams, or patients, we craft the story and approach to reach your audience. We work with all clients, from Fortune 500 pharma companies, start-ups, and non-profits in a wide array of therapeutic areas.Writing services include publications (manuscripts, posters), competitive intelligence, medical conferences, product support (slide decks, SRLs, literature searches), advisory boards, educational materials for physicians and MSLs, training modules for sales and MSL teams, and academic books and book chapters.Please message me to inquire about availability.

•Nation-wide physician liaison•Authored and presented papers and posters for publication•Edited creative and publications for scientific rigor and brand compliance•Collaborated with scientific advisory board to develop clinical study methodologies for grants•Developed campaigns and materials for education, PR, and marketingAchievements:•Brought in initial physician and patient customers•2018 SBIR grant application project lead•Designed and implemented educational and sales outreach to physicians and patients•Created and carried out clinical training programs for physicians and staff•Developed all initial materials, website, and social media•Developed and ran business operations

-Collaborated with clients and key opinion leaders to optimize digital marketing strategy and develop timely, quality content for clients- Supported account leads on all projects- Managed internal team on projects- Ensured client expectations are met and managed- Edited projects for quality assurance

-Collaborated with clients and key opinion leaders to optimize medical strategy and develop timely, quality scientific and clinical content for pharmaceutical and non-profit organizations- Scientific editorial writing included (but was not limited to) slide kits, data mining, virtual advisory board meeting preparations, video scripts, review articles, newsletters- Client counsel included establishing/maintaining relationship of trust with clients, identifying unmet needs, disease and cause marketing, and offering professional/technical leadership across multiple diverse projects

The amyloid-beta peptide accumulates in the brain of Alzheimer’s disease patients and initiates disease onset. Our lab studies the cellular pathways that regulate the formation and clearance of amyloid-beta. I am the lead technician performing in vivo microdialysis in mouse models of AD; this technique enables us to study how amyloid-beta changes every hour over several days in response to genetic, drug, or behavioral manipulation. The amyloid-beta microdialysis technique was originally developed in our lab and is only used by 7 other groups in the world; including Case Western Reserve and various other universities who visited Washington University so I could teach them this technique. In addition to performing our own experiments, I am also responsible for organizing, coordinating, and executing research projects in collaboration with academic and corporate groups. Techniques used: In vivo Aβ microdialysis, small animal stereotaxic surgery, intracerebral injections, drug administration to mice, sandwich ELISA, custom construction of Electroencephalography probes, and data analysis.

Huntington’s disease derives from a polyglutamine expansion in the huntingtin gene. This expansion results in the misfolding and aggregation of the protein in the cell where it becomes neurotoxic. Accurate quantification of the protein in in vitro is difficult. I constructed a huntingtin protein with a Cyc-Cys-Gly-Gly motif attached to either the amino or the carboxyl terminal of the gene so that aggregates could then be labeled using the biarsenical reagent FlAsH-EDT2. This reagent binds only to a tetracysteine motif which the huntingtin aggregates reconstituted, coordinating with FlAsH-EDT2 and fluorescing. Wild type huntingtin, which does not aggregate, did not fluoresce. Techniques used: PCR, ligation, designed primer, gel electrophoresis, bacterial transformation, plasmid purification, transfection, cell culture, western blot,

The Human Microbiome Project is a study to develop a reference of the microbial genome sequences that colonize the human body. The goal of the reference is to learn what microbes are present and colonizing in both health and disease states in order to better personalize medicine and nutrition. The microbes are identified by sequencing the 16s ribosomal RNA, chosen for its stability and conservation between different species of bacteria and Achaea. I prepared samples for sequencing through amplification and purification of samples, which I then used in transforming E. coli competent cells. Techniques used: PCR, gel electrophoresis, DNA purification, DNA ligation, electroporation.