Danielle Lesperance Email & Phone Number

Boston, Ma, Us

Watertown, Ma, Us

● Project lead for multiple customer pipelines in Microbiome Process Development at ReciBioPharm (Formerly Arranta Bio)● Responsible for designing and troubleshooting bench and pilot scale bacterial fermentation and TFF experiments for development of live biotherapeutic products● Trained in upstream cGMP biologics production● Supported tech transfer activities to define bioprocessing and harvest workflows and schedule critical process points● Generated technical reports detailing process development work and results following FDA guidelines for ease of customer IND filing● Gained knowledge of CMC considerations in drug development

Watertown, Ma, Us

● Scientific project lead supporting upstream development of microbial strains as live biotherapeutics ● SME in cultivation, fermentation, harvest, and lyophilization of aerobic and anaerobic microbes● Prepared documentation including development plans, batch records, and SOPs● Trained junior personnel in proper aseptic technique and microbiology practices

Baltimore, Md, Us

● Utilized computational approaches to analyze next generation sequencing (NGS) data from microbiome samples● Authored SOPs for common and specialized laboratory practices● Conceptualized and generated a comprehensive laboratory wiki site for centralized organization of shared materials

Storrs, Ct, Us

● Managed multiple projects investigating host-microbe interactions using an insect system● Conceptualized in vitro, in vivo, and ex vivo methodologies for microbiome analysis● Developed technical proficiencies in aseptic technique and aerobic cultivation of microbes● Mentored junior graduate students and undergraduates in technical laboratory skills and management of independent projects● Routinely presented results and analyses to internal and external audiencesAdditional Activities:• Reviewed manuscripts for Cell Reports and Current Opinion in Insect Science• Organized a public movie screening of the documentary Resistance followed by a discussion panel of antibiotic resistance experts in conjunction with the Center for Disease Control’s World Antibiotic Awareness Week

Storrs, Ct, Us

Courses: “Characterization of Microbial Communities by 16S rRNA Gene Sequencing” Module (2019, 2020), MCB-PSM Microbiome Workshop (2018, 2019)• Prepared and presented lectures, designed syllabi, worksheets, and exams, and supervised laboratory sessions for courses introducing 16S rRNA microbiome sequencing

Storrs, Ct, Us

Courses: Tiny Earth Instructor Training Workshop (2018, 2019), Small World Initiative Partner Instructor Training Workshop (2016, 2017), “Fundamentals of Microbiology” Laboratory (2015-2016)• Supervised laboratory sections and prepared and presented lectures on microbiology concepts and techniques

● Optimized assay for testing immunotherapeutic candidates for their effectiveness as diabetes therapies● Identified multiple biotherapeutic candidates to test for efficacy in vitro and in vivo● Managed lab operations including inventory, equipment maintenance, and coordination with collaborators

Boston, Massachusetts, Us

● Identified and published a cell phenotypes-switching pathway in pancreatic islets using mice and tissue culture models● Utilized fundamental molecular biology techniques for analysis of gene expression, protein quantification, and cell sorting

Amherst, Massachusetts, Us

•Utilized molecular cloning and cell culture techniques to aid senior graduate student in exploring potential vaccine vectors for Chlamydia trachomatis prevention

Hartford, Ct, Us

•Communicated with and collected information and body measurements from patients in Intensive Care

Doctor Of Philosophy (Phd), Molecular And Cell Biology

Bachelor Of Science (B.S.), Molecular And Cell Biology