Part of the Tumor Immunotherapy and Microenvironment (TIME) group (PI: Dr. Bassam Janji). Evaluation of the impact of autophagy modulating small molecules (Vps43 inhibitors) on tumor progression and immune cell functions within the tumor micro-environment in vitro and in vivo in combination with immune-checkpoint inhibitors (anti-PD1 and anti-PD-L1 MAbs) using B16-F10 and CT26 syngeneic mice models. In-depth investigation of drug effect by flow cytometry and CyTOF technology.

Coordinating and managing a team composed of two lab technicians and a research associate. Project dependent management of collaborators outside of my team. Supervising the successful progression of multiple projects in parallel. Other tasks were immune cell-based assay development, data analysis, reviewing and presentation to clients, drafting of project proposals for potential clients.

Working on interdisciplinary projects for and with leading pharmaceutical companies and biotechs, advancing drug discovery projects at various stages through contribution of keyexperiments (conception, execution and analysis) within the in vitro immuno-inflammation/oncology group. Key tasks included developing assays on cell lines (THP1, Jurkat cells) and primary immune cells: co-culture assays (MLR, CD8 Killing and Treg suppression assays), macrophage/DC differentiation, T cell and… Show more Working on interdisciplinary projects for and with leading pharmaceutical companies and biotechs, advancing drug discovery projects at various stages through contribution of keyexperiments (conception, execution and analysis) within the in vitro immuno-inflammation/oncology group. Key tasks included developing assays on cell lines (THP1, Jurkat cells) and primary immune cells: co-culture assays (MLR, CD8 Killing and Treg suppression assays), macrophage/DC differentiation, T cell and monocytes activation. The overall aim within most projects was the validation of therapeutic targets as well as demonstrating the MoA and the efficacy of lead compounds through functional assays (semi-automated compound profiling in 96 and 384-well format) using state of the art techniques (flow cytometry, HTRF, MSD technology) as well as preparing and presenting the data to a diversified audience (internally and externally) in a clear and concise manner. Show less

Employed in the frame of the Graphene flagship at the “Institut de Biologie Moléculaire et Cellulaire” (IBMC) on "2D and carbon multifunctional nanomaterials for therapy" (PI: Dr. A. Bianco). Evaluation of the impact of few layer graphene on immune cell functions in vitro and in vivo with a special focus on autophagic functions and the use of graphene oxide to mediate siRNA transfection in vitro. Substantial expertise gained in gene silencing, nanomaterial toxicity evaluation, maintenance of… Show more Employed in the frame of the Graphene flagship at the “Institut de Biologie Moléculaire et Cellulaire” (IBMC) on "2D and carbon multifunctional nanomaterials for therapy" (PI: Dr. A. Bianco). Evaluation of the impact of few layer graphene on immune cell functions in vitro and in vivo with a special focus on autophagic functions and the use of graphene oxide to mediate siRNA transfection in vitro. Substantial expertise gained in gene silencing, nanomaterial toxicity evaluation, maintenance of diverse cell lines (HeLa, HEK T293, RAW 264.7) and in imaging by confocal microscopy. Show less

PhD student under the supervision of Pr. Sylviane Muller and Dr Frédéric Gros. My research was focused on understanding the role of macroautophagy in B and T cell biology in normal and autoimmune conditions. This included the generation of KO mice models and the development of adapted immuno-assays. Excellent skills were acquired in the care and monitoring of mice strains, flow cytometry, immune cell isolation, primary cell culture, immunoblotting, ELISA and basic molecular biology (primer… Show more PhD student under the supervision of Pr. Sylviane Muller and Dr Frédéric Gros. My research was focused on understanding the role of macroautophagy in B and T cell biology in normal and autoimmune conditions. This included the generation of KO mice models and the development of adapted immuno-assays. Excellent skills were acquired in the care and monitoring of mice strains, flow cytometry, immune cell isolation, primary cell culture, immunoblotting, ELISA and basic molecular biology (primer design, PCR, RT qPCR). Show less