Scott Donovan Email & Phone Number

Carlsbad, California, United States

Cambridge, Massachusetts, United States

Cambridge, Massachusetts, United States

Cambridge. Ma

Study translation of mRNA

Study the translation of mRNA

85 Wells Ave Newton Ma

Scientist who is investigating mechanism of actin of unique pre clinical drugs.

Cambridge Ma

• Maintain proper stocks of chemicals in Lab.• Set up and maintain all Fluidigm devices at Harvard.• Assist researches in planning and executing Fluidigm Projects.• Assist researchers in analyzing data from Fluidigm Projects. • Carry out independent Fluidigm experiments.• Create Nextera Libraries for single cell sequencing• Create RNA libraries using the Illumina Trueseq Kit• Extract and purify DNA – Qiagen mini-, midi- and maxi-preps.• Passage and maintenance of cell culture lines• Clone crisper cast9 into bacterial cells Preform fRIP to determine chromatin regulators in RNA

Troubleshoot and improve existing SOPsValidate Fluidigm Single cell technology Develop in House Single Cell and RNA Assays Develop new protocols of emerging Gap Technologies Develop and implement new SOPs Evaluate and improve current Gap protocols Evaluate if new technologies will be viable In GAP Program EVO, Multi probe II, Bravo, Beckman Robotics.

• Prepare samples for ABI and Solexa sequencing.• Check the quality of all PCR reactions before hand-off.• Developing and programming scripts for multiple robotic platforms.• Primer design and verification.• WGA DNA• Development on quantification and normalization processes.• Process development for Luminex.• Prepare RNA samples for expression run on Luminex.• Assist in the maintenance of Luminex scanners.• Analyze Luminex data using Excel.• Design and implement protocol.

• Organize and maintain all lab instrumentation• Extract and purify DNA – Qiagen mini-, midi- and maxi-preps• Prepare bacterial and mammalian culture media• Passage and maintenance cell culture• Analyze DNA through SDS page, electrophoresis, and restriction digest• Analyze proteins through Western Blot• Maintain a laboratory notebook• Assist scientists with lab experiments• Schedule global project team meetings• Prepare Capital acquisition requisitions• Perform data and journal searches

• Prepare/analyze tissue samples by immunohistochemistry; prepare fixative to archive and store samples• Small animal training – handling of rodent species, employing techniques of perfusion and CNS dissection• Establish repertoire of antibody specificity in support of immunohistochemistry applications• Perform data and journal searches