Doug Bisson Email & Phone Number

San Diego, Ca, Us

Lexington, Massachusetts, Us

Lexington, Massachusetts, Us

• • Planned and operated fermentations of multiple organisms (E. coli, S. cerevisiae, P. pastoris) at 1L and 5L scales for production of UGT enzymes and natural product small molecules • Vastly improved product titers in both the enzyme and natural product platforms through process improvements, strain selection, and media optimization • Generated material for molecular analysis, animal trials, and partnership agreements• Isolated and purified fermentation broth of multiple natural product molecules to be utilized in animal model studies• Expanded fermentation capacity by leading the capital purchase of new equipment• Developed HPLC method to analyze fermentation broth sugars and byproducts

• Expanded and improved fermentation lab by sizing, purchasing, and installing new bioreactors, autoclave, air compressor, recirculating chiller, and steam generator.• Designed, set up, and operated weekly E. coli fermentations (2L, 15L) for production of multiple types of HMOs (Human Milk Oligosaccharides). • Produced gram and kilogram quantities of HMOs for analytical assays, animal model trials, patent documentation, and business partner examination.• Improved a research-scale process through strain selection, media and process optimization, and DOE, in order to enhance product titers, yield, productivity, purity, and scalability.• Scaled up fermentation processes for domestic and international CMOs through tech transfer documentation, on-site training and supervision, and regular remote check-ins.• Trained and managed a direct report in all aspects of the fermentation process and related responsibilities.• Analyzed results in individual fermentation, weekly, and strain-based reports, and presented data at bi-weekly company meetings to update downstream, microbial, and analytical team members.• Created SOPs for the in-house fermentation process and all supporting activities, as well as scalable SOPs for CMOs.• Maintained all fermentation reactors and lab equipment via preventative maintenance, repair, and replacement of parts.

Woburn, Massachusetts, Us

• Operated weekly E. coli fermentations in their production of PHA natural plastics through manual and computerized monitoring• Improved fermentation performance through strain selection, media optimization, and process advancements to meet team and process goals• Successfully scaled-up and transferred technology from in-house 20 liter lab fermentors to four pilot-sized fermentation sites of various sizes• Supplied material for product formulation, initial customer testing, and final customer products from these pilot-scale fermentations• Troubleshot multiple offsite facilities that developed difficulty with running the process and determined the cause of the problem resulting in successful fermentations• Developed flocculation technology for high cell density culture, increasing cell settling time from days to minutes• Created SOPs for entire team for fermentor command programs, various summary reports, and equipment operation• Involved in all aspects of fermentor repair and maintenance including troubleshooting, inspection, CIP, SIP, and component replacement

Ms, Biotechnology - Engineering

Bs, Chemical Engineering