Aswini Krishnan (Dr. Rer. Nat) Email & Phone Number

India

Greater Zurich Area

- Cross-functional collaboration with University Hospital Zurich (USZ) and drug screening platform (Nexus, ETH), to plan project strategies and execute project goals- Project co-ordination and management involving patient sample screening and acquisition - Data management and statistical analysis of clinical data for the evaluation of prognostic outcomes- Optimize pre-drug screening processes, estimate and ensure material availability for planned experiment- Contribute to reviewing, editing and submission of scientific reports- Develop and present scientific data updates/progress to diverse audience

Mainz Area, Germany

- Genomic and proteomic analysis of normal vs tumor vs metastatic patient tissues with Papillary Thyroid Cancer (PTC)- Validation of identified genomic and proteomic candidates as oncogenic alteration and/or therapeutic targets through extensive cell biological and biochemical assays- The study identified and validated a novel oncogenic fusion, as well as proteins involved in the Ubiquitination pathway as potential therapeutic markers in PTC- Cell banking of patient derived cells- Documentation of lab activity and data- Provide training and supervision to team members

Bangalore

- Establishment and maintenance of the Cell Culture Laboratory- Establishment of primary neuron culture from rat embryo brains- Identification of mRNA targets of FMRP amygdala and hippocampus of one month old rats

Beatson Institute For Cancer Research, Glasgow

1. Scientific officer, Urology Research Laboratory, Supervisor Prof. Hing Leung. (June 2011 – February 2012)- Investigation of centrosome kinase SIK2 as a novel therapeutic target for prostate cancer- Study the phenotypic cellular effects of SIK2 knockdown using siRNA in various prostate cancer cell lines2. Scientific officer, Ubiquitin Signalling Laboratory, Supervisor Dr. Danny Huang. (November 2010 – May 2011)- Expression (in E.coli), purification and assembly of protein for crystallisation studies

The Kroto Research Institutenorth Campus, University Of Sheffield, S3 7Hq

- ELISA based quantification of interleukins (IL-8, IL-6 and IL-1α) released by keratinocytes and fibroblast cells maintained in different types of conditioned media.

Kroto Research Institute

Project title: Investigation of the ability of collagen IV in the enhancement of neuronal cell growthMy project focussed on the effect of different biomaterial surfaces on the adsorption and subsequent functionality with respect to cell attachment and proliferation of the extra cellular matrix protein collagen IV. Quantification studies for the adsorption of collagen IV on biomaterial surfaces glass, PLLA and acrylic acid using ELISA showed that there was no significant difference in the adsorption of collagen IV among the surfaces investigated. F-actin staining studies using confocal microscopy in NG-108 cells indicated that there was no appreciable difference in the attachment of cells on the different surfaces. Also, the dependence of cell attachment on beta1 integrin, a high affinity receptor for collagen IV was investigated by integrin blocking studies. This study implicated the role of beta-1 integrin on cell attachment. The cell number analysis studies suggested that collagen IV coating had no significant effect on cell proliferation.

Ph.D, Oncology And Cancer Biology, Magna Cum Laude (1.0)

Master'S Degree, Stem Cell And Regenerative Medicine

Obtained International Student Scholarship 2008-2009, University of Sheffield

Bachelor'S Degree, Biotechnology, Chemistry, Botany