Ganesh Thapa Email & Phone Number

County Limerick, Ireland

Dublin, Ireland

To advise Trinity research family and oversee the broader safety management programme. To provide professional expertise in areas relating to the control of biological hazards across Trinity arising from work with all types of biological agents including GMOs and infectious dangerous pathogens. To ensure the development of policies and procedures to meet with legislative requirements and the highest industry standards and its implementation in Trinity research community.

University Of Limerick

Protein and Peptide research, Functional peptides & food, bio active peptides, diabetics and Cancer

County Dublin, Ireland

I worked as lead scientist in TILLING (Targeting Induced Local Lesions in Genomes) project leading to develop future wheat with enhanced yield and sustainability for hostile environment. The identified SNPs were Kompetitive allele specific PCR (KASP) and High Resolution Melting (HRM) validated and associated with disease assessment and phenotypic data. I also led two other projects (a) Phosphoproteomics interaction project, wherein possible effect of SNP in BRI1 kinase domain on downstream signalling was dissected upon. (b) Cloning and Functional validation of leucine rich repeat receptor kinase for its role in Fusarium resistance through VIGS (Virus induced gene silencing), expression and signalling analysis. I supervised a team of 23 professionals including Marie-Curie ITN PhD, Masters and undergrad students. I led review meeting that look into the Strategic and scientific hypothesis, its rationale, providing full support on areas such as planning, design and realistic scientific accomplishments, future experiments accuracy, supervision & training, Lab aseptic and equipment review, Lab ordering/POs, Collaborators networking, Lab safety & Occupational Health, project review report, SOEs/SOPs, Ethics and dissemination and Resourcing.I could successfully complete all of the 3 projects, developed resource of 2016 GENOME EDITED validated lines of BRI1 receptor kinase snd revealed differential regulation of BRI1 signaling due to SNP in BRI1 kinase domain. I validated the function of LRR-receptor kinase by reverse genetics approach and got European patent application no.: EP17160440.8 for first wheat Fusarium resistance LRR receptor kinase. I successfully disseminated and published papers in peer high impact factor journals. I managed team of diverse culture and background. I established strict GMO guidance of aseptic rules, ISO17025 and applicable GMP. I introduced a new inventory and lab Management System for quick access to chemicals and stocks.

Role Overview:• Working as main lead scientist of the research project entitled “Expression of N15 labelled HIV1 protease for development of drugs” and to train the technicians, students, interact with collaborators and manage lab logistics to achieve the scientific goal• I need to bioengineered molecular expression vector for the N15 labelled wildtype along with mutant variants of HIV-1 protease from Multi Drug resistance (MDR) patients. Once expressed, the HIV-1 protease was needed to be purified and structurally characterized by NMR, IR spectroscopy, MALDI-TOFF and LC-MS to reach at possible functional inhibitors to facilitate drugs development, even for MDR patients. • My role also incorporated a framework of Genetic manipulated organisms (GMO) protocols and training, experimental Planning, Graduate & post grad teaching, lab management, lab review meeting, supervision and dissemination. • Supervise and guide a team of project fellows and masters/undergrad students for their relevant thesis research workKey Achievements:• Secured 0.5 M RANDS Grant funding for this UKZN-PDF project in 2013.• I successfully tackle the nagging problem of expressed proteins going into inclusion bodies by adjusting the culture parameters and even successfully harvesting inclusion bodies to rescue complete expressed HIV-1 protease.• I formulated and successfully designed the scientific strategy while keeping in mind of learning focus of students to expressed and purify N15 labelled recombinant HIV-1 protease. The N15 labelled HIV-1 protease was purified and characterized by MALDI-TOFF and LC-MS for inhibitor development• I developed my skills of proteomics, protein structural characterization, enzyme assay and bioengineering techniques.• Undergone GMO training under strict guidance of aseptic rules, ISO17025 and GMP guidelines.

Guwahati Area, India

My Phd project involved cloning and functional characterization of heavy metal stress responsive gene from Eichhornia crassipes. The Echmr gene was cloned and found to provide multiple resistance to heavy metals and other abiotic stresses as found in gsh mutants and ycf1 mutants in bacteria and yeast respectively. I also characterized abiotic stress genes SnRK2.8 and Pyl13 from Aarabidopsis while supervising my two masters students. I had also undertaken teaching assignment for M.Tech and B. Tech courses. I also successfully supervised the research projects of 3 B. Tech students and 3 M. Tech students during this period.

Jorhat, Assam, India

Reporting to the Principal Investigator and working as part of the Research project Team. I was responsible for collection of samples, identification, maintenance and conducting lab research work.My areas of responsibility include Project progress report, Resourcing, lab safety & Occupational Health, lab order Management, paper writing and TrainingKey Achievements:• Setup tea clones’ resource of Assam and marked it as water logging resistant. • Successfully developed simple sequence repeat (SSR) markers and verified all collected clones.• Finished project within timelines and purchased high end equipment such as GC, LC-MS, Realtime PCR for the lab.

New Delhi, Delhi, India

I was responsible for all lab research activities associated with the research project “Identification of the salinity tolerance inducible genes in rice” in the Department of Plant Molecular Biology, University of Delhi, South Campus, India. This involved (a) Functional characterization of specific salt stress upregulated/downregulated (ESTs)/clones identified by using Reverse Northern and conventional Northern hybridization and subsequent isolation and characterization of their promoters. (b) Analysis of transgenic rice plants over-expressing two different heat shock proteins and raising them to homogeneity. (c) Isolation & characterization of root specific promoters and cloning in binary vector pCambia1381Z with the uidA gene. (d) Molecular biological analyses of Brassica plants over- expressing the tobacco basic 1,3 chitinase gene and bioassays of the transgenics using Alternaria brassiceae to check their fungal resistance. • I had full responsibility for activities relating to research work in lab, Greenhouse, Bioassay experiments, lab orders Management, Training, Radioactive experiments.Key Achievements:• Successfully created Suppression Subtraction Hybridization (SSH) salt stress cDNA library, identified several promising salt stress associated genes like UBC4, eIF2α, PPiase, E3 Ubiquitin ligases and validated in corresponding yeast mutants. These genes were functionally validated by overexpression in rice and tobacco plants. • Characterized pyramid HSP100 and HSP18 transgenic lines. I isolated root specific promoter from rice and analyzed it by promoter-GUS assay and EMSA. Brassica overexpressing lines were also phenotyped and characterized for fungal resistance. I developed and learned advance skills of molecular biology and learn time and research management of multiple projects.

Jorhat, Assam, India

Role Overview:• Reporting to the Principal Investigator and working as part of the research Project Team of a project “Identification of molecular marker linked to gene for submergence tolerance in rice” which involved random amplification of polymorphic DNA (RAPD) analysis and generation of sequenced characterized amplified region (SCAR) primers, and statistical analysis of the results obtained. • I had full responsibility and autonomy for overseeing the research management and expenses, training & teaching, lab health & safety, progress report writing, lab orders and project work dissemination.Key Achievements:• Several submergence tolerant and susceptible rice cultivars were characterized and specific RAPD primer has been developed for tolerant cultivars. Research work disseminated and published. Develop SCAR marker for submergence tolerance.• Encouraged greater levels of accountability and research linkage across students and technicians.

Jorhat, Assam, India

Role Overview:• Reporting to the Principal Investigator and working as part of the research Project Team of a project entitled “Decomposition of tea organic waste to value added compost”. • Duties included lab work, training & planning, lab health & safety, project work report and dissemination.Key Achievements:• This work has led to the identification and functional characterization of compost- degrading strains of bacteria and fungus.

Associate Degree, Prince 2 Project Management

Activities and Societies: project trigger, preproject, themes, project, processes, decision making, directing a project, stages, project.

Professional Diploma In Health & Safety, Health And Enviroment Safety, Biohazards And Biosecurity, A

Behavior Based Safety, Accident Investigation, Health & Safety: Legislative Context, Health & Safety Management System Integration.

Microsoft Power Bi, Business Artificial Intilliegence And Data Analytics, Visualization, Decision Making

Activities and Societies: Data preparation, modeling, visualization, analysis, asset deployment and maintenance, Power Query, Pivot table.

Google Data Analytics Professional Certificate, Data Analytics, 9.7

Activities and Societies: Job portfolio data collection, preparation, analysis, spreadsheets, SQL, presentation tools, Tableau, RStudio.

Associate Of Science - As, Process Data Analytics

Process Data Analysis with R, Regression Analysis, Data Mining and Visualization, Data Analytics Case Study, Introduction to Data Analytics

Doctor Of Philosophy (Phd), Bioengineering & Biotechnology, 8.9

Activities and Societies: Research, soccer, Bioinfo, visit to RedCross orphanageDiscovery and validation of a sustainable phytoremediation.

Master'S Degree, Biotechnology, Merit

Activities and Societies: Biotechnology Society of India, ISAB, ISBDevelopment of genetic markers to preserve the tea clones of Assam..

Bachelor'S Degree, Agricultural And Plant Breeding-Genetics, 1.1 & Distinction

Activities and Societies: Mushroom farming, football, NSS, Study India tripHONS in plant breeding and genetics

10Th And 12Th, Pcmb, Merit & Distinction