Dr.Nasrin Begam Email and Phone Number

Task• Planning and execution of R&D projects (Upstream and Downsteam) at lab scale • Identification and sourcing of chemicals, consumable and reagents required for R&D projects. • Following up with SCM team for procurement of chemicals, consumable and reagents • Working with ARD team to establish the specifications for assessing in-process and finished products. • Monitoring and follow up IBSC, RCGM and Committee Member of IBSC

Achievements/Tasks• Planning and execution of R&D projects.• Monitoring and follow up IBSC, RCGM.Project Involved • LiraglutideExecution of bacterial fermentation, Purification, and Acylation of the recombinant Liraglutide • L AsparaginaseProcess modification and development to get maximum purity and activity of L Asparaginase• Pegylated AsparaginaseProcess development of the Pegylation reaction of Asparaginase to produce stable potent Peg Asparaginase.

NGS data analysis

The project undertakes work on identification of epigenetic factors in early detection of breast cancer. Adequately designed primers were used to distinguish between methylated and unmethylated genes by Methyl specific PCR. mRNA expression analysis of ATM,TP53,GSTP1,APC and TIMP3 by RT PCR was also done. The findings indicated that there was hypermethylation of promoters of ATM,APC,TIMP3 and GSTP1 and this could serve as biomarker in distinguishing the cancerous from non-cancerous cells. Also, hypermethylation of three of these, namely, ATM, APC and GSTP1, was associated with aggressive form of cancer lymph node positive phenotype and metastasis. The ATM and APC promoters were suggested to be useful as biomarkers. Correlation with age was also observed and the data was statistically found to be significant.This work leads to my PhD degree with 3 research publications in peer reviewed journals and 3 conference presentations.

From large collection of cultivated denitrifiers, I recovered culture from liquid N2 storage and cultivate them using standard microbiological technique. I also helped to isolate additional denitrifier strains from sediment samples, in order to add them collection. I isolated DNA from the cultures and screened them for the presence of nir S gene (encoding nitrate reductase ) using the polymerase chain reaction (PCR).I also helped to extract DNA from sediment samples, which is more challenging than extracting DNA from cultures. I performed several different PCR assay on these samples. In addition, I helped run some nutrient assays, measuring inorganic nitrogen concentration in seawater samples using spectrophotometric methods.

I was assign to the project on "Induced Mutagenesis in Black cumin(Nigella sativa L.)" for crop improvement. During the period I help to identify mutagen induced anomally in Nigella sativa L.by morphological and cytological study of F1 generation. I also learn breeding technique

I have done extraction of plant secondary metabolites from the leaves of Tamarindus indica and Purification by column chromatography. Rice seedling bio-assay along with Anti fungal & antibacterial activity was also done. This work is published in Annals of Tropical Research