I am Rakesh Kumar, recently in 2023, I did my Ph.D. in Biotechnology from Maharshi Dayanand University, Rohtak on the topic entitled “Molecular characterization and development of diagnostics for Onion yellow dwarf virus associated with Onion (Allium cepa L.) crop in India” under the supervision of Dr. V.K Baranwal, National Professor, ICAR-IARI, Delhi and Dr. Sonia Kapoor, Professor, UIET, MDU, Rohtak, Haryana Presently, I am working as “Senior Project Associate” at Division of Plant… Show more I am Rakesh Kumar, recently in 2023, I did my Ph.D. in Biotechnology from Maharshi Dayanand University, Rohtak on the topic entitled “Molecular characterization and development of diagnostics for Onion yellow dwarf virus associated with Onion (Allium cepa L.) crop in India” under the supervision of Dr. V.K Baranwal, National Professor, ICAR-IARI, Delhi and Dr. Sonia Kapoor, Professor, UIET, MDU, Rohtak, Haryana Presently, I am working as “Senior Project Associate” at Division of Plant Pathology, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, India and carrying out research on High throughput sequencing (HTS) based identification, molecular characterization, development of nucleic acid and serological based diagnostics for identification of viruses/viroids associated with different horticultural and vegetables crops. I have total 10 years of experience which includes more than 7 years of experience in plant molecular virology and 3 years of expertise in molecular nematology research. I am well versed with numerous molecular biology techniques which includes PCR, qRT-PCR, multiplex PCR, Immunocapture PCR, RPA assay, protein expression, extraction and purification, SDS-PAGE, western blotting, DAC- and DAS-ELISA, RNAi construct designing, gateway cloning, plant tissue culture techniques, transgenic development, database and bioinformatics tools i.e. NCBI, EXPASY, ClustalW, Bioedit, MEGA X, RDP 4, SDT, ORF finder, Primer 3 and IDT etc. Show less