o Analysis of the cardboard for cellulose, hemicellulose and lignin content (ASTM std with HPLC)o Process development for physical size reduction with hammer millo Design of experiments (DOE) for pretreatment based on alkali and ozone for the removal of lignin from cardboard wasteo Optimization of the pretreatment process based on glucose yield and cost analysis from the model generated by DOEo Measurement of enzyme cellulase activity with FPU assay and enzyme β glucosidase activity with pNPG assayo DOE for enzymatic hydrolysis based on combination of mix enzyme o Analysis for glucose and hemicellulose content with HPLCo Optimization of the enzymatic hydrolysis process o Expansion of Burkholderia cepacia on agar plate for preparation of inoculum, nutrient brotho Media optimization to multiply and grow B.Cepacia for the production of biodegradable polymer P3HB.o Optimization to get longer chain of PHB with optimum nitrogen to carbon ratio (N:C) with time. Working with small scale bioreactors following aseptic techniqueso Process to harvest PHB from bacterial cell, purification of PHB and analysis of PHB with 1H NMR, TGA, FT-IR, TEM, GPCTeaching Assistant-o Assist university professor and faculty members in teaching and research activities at UMLo Organize reference materials, visual aids and other materials as required by professors for lectureso Daily group meeting, conduct seminars, discussion on selected topics and laboratory sessions to supplement lectureso Assist in the preparation and administration of examinationso Grade examinations, term papers and laboratory reportso Conduct literature reviews, surveys, laboratory experiments and other research for use in scholarly publicationso Supervise and train the other junior research fellows in the group for laboratory experimentso Advice the incoming new student joining the BMEBT program at UML, regarding the selection of the specialization subjects that matches their background