Ajinkya Pattekar Email & Phone Number

Philadelphia, Pennsylvania, United States

• Responsible for running the product characterization assays to look at quality of the product and its response to antigen stimulus including in-vitro culuture, spectral flow cytometry, single cell library generation.
• Processing the patient tissue samples and evaluating the immune response through 10x single cell FLEX library generation and spectral flow cytometry.
• Analysing the data generated and presenting it in company-wide meetings.
• Optimizing, validating, troubleshooting new assays/ protocols that can be incorporated in the workflows like 10x visium, Isolight. This includes collaboration with CROs and FAS from the respective companies.

Philadelphia, Pennsylvania, United States

Philadelphia, Pennsylvania, United States

• Develop, Optimize, integrate immunological assays including flow cytometry and CYTOF to help understand immunologic answers from the patient samples with a goal of optimizing clinical care.
• Built a sample processing unit that collects and processes clinical biospecimens for scientific research.
• Designed protocols for isolating live single cells from blood and tissue in a BSL-2 enhanced environment.
• Mentored over 10 technicians and taught scientific best practices.
• Lead a team of 6 to deliver high quality single-cell biospecimens for dozens of research groups.
• Validated and operationalized new technologies for high-throughput immune profiling

Greater Philadelphia Area

• IBD Project.
• Processed the Intestinal biopsies and blood from patients to isolate single cells.
• Responsible for freezing and storage of the cells in appropriate conditions.
• Maintained inventory of all the stored samples for the ease of further analysis.
• Conjugated Antibodies to metals and maintained the antibody inventory.
• Planned and performed stainings of about 900 samples across 2 different panels.

Greater Philadelphia Area

• Preclinical Testing of novel immunotherapy for HTLV-1 induced diseases.
• Observing the co-expression patterns of inhibitory receptor expressions and devising a blockade strategy against these receptors.
• Performed IHC staining on mouse tissue sections. Captured pictures with Bright field microscope and Confocal microscope.
• Edited and reviewed manuscript for publications.Apigenin as a potential therapy in neuroinflammatory diseases.
• Testing the potential of Apigenin to reduce neuroinflammation by reducing the dendritic cell (DC) migration to the CNS.
• Separated PBMCs from buffy coat followed by DC isolation and subsequent treatments with proinflammatory agents and Apigenin.

Greater Philadelphia Area

• Preclinical projects Lab of Dr. Michele Kutzler, PhD
• Vaccines for Clostridium Difficile: A unique DNA plasmid encoding the receptor binding domain of toxins A and B has been generated and tested for efficacy in vivo. My role has been to assist with Immunizing mice.
• Vaccines for HIV: The development of mucosal targeting molecular adjuvants has been recognized as an innovative means to induce anti-HIV mucosal host immunity when co-delivered with HIV-1 DNA-based vaccine antigens. My.

Philadelphia

Clinical: Patient specimen processing for clinical research projects including sera, plasma and PBMC purification from whole blood, stool processing for mucosal antibody analysis, writing of laboratory standard operating procedures, storage and record keeping of specimens. Molecular and cell biology: Cell culture, SDS-PAGE, western blot, ELISA, ELISPOT.

Mumbai

Clinical experience (MD program and Internship)Providing medical and surgical care for Inpatients and Outpatients.

Master’S Degree, Immunology, 3.74

Doctor Of Medicine (M.D.), Medicine, 3.1 Gpa

Six year degree program. GPA: 3.1