Muhammad Akbar Shahid

Muhammad Akbar Shahid Email and Phone Number

Assistant Professor at Bahauddin Zakariya University, Multan @ Bahauddin Zakariya University, Multan
multan, punjab, pakistan
Muhammad Akbar Shahid's Location
Multan District, Punjab, Pakistan, Pakistan
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About Muhammad Akbar Shahid

I did DVM (Doctor of Veterinary Medicine) and Masters in Veterinary Microbiology from University of Agriculture, Faisalabad, Pakistan in 2004 and 2006, respectively. I remain involved in poultry disease diagnostics for more than two years in Poultry Research Institute, Rawalpindi, Pakistan. I was awarded a highly prestigious scholarship by Higher Education Commission (HEC), Pakistan to complete PhD from the University of Melbourne, Australia in 2014. My PhD project entitled "Molecular basis of attenuation of Mycoplasma synoviae” encompassed comparative genomics, functional genomics and proteomic studies to underpin the pathogenicity determinants of an economically important poultry pathogen. My PhD research was focused on understanding the genetic factors that rendered the MS-H strain, the only commercially available temperature-sensitive vaccine, avirulent. Complete genome sequences of various M. synoviae strains, differing in pathogenicity and tissue tropism, were obtained using high throughput next generation DNA sequencing technologies (SOLiD and Illumina) and compared to discover the putative genetic lesions. For the first time, plasmid based expression vectors were developed and used to genetically manipulate this mycoplasma. I characterised the role of missense mutation(s), mapped in an essential GTP binding protein Obg, implicated in temperature sensitivity phenotype of MS-H strain. I also developed a number of molecular tools for rapid diagnosis and differentiation of the vaccine strain from field strains of M. synoviae. My PhD research work has provided basis for the development of novel recombinant vaccines for M. synoviae. I published doctoral research work in international, peer reviewed, impact factor journals and also presented research findings in international conferences held in Australia and France. My aim is to join academia, in the field of microbiology, to train more manpower and to enhance quality of education and research.

Muhammad Akbar Shahid's Current Company Details
Bahauddin Zakariya University, Multan

Bahauddin Zakariya University, Multan

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Assistant Professor at Bahauddin Zakariya University, Multan
multan, punjab, pakistan
Website:
bzu.edu.pk
Employees:
418
Muhammad Akbar Shahid Work Experience Details
  • Bahauddin Zakariya University, Multan
    Assistant Professor
    Bahauddin Zakariya University, Multan Mar 2018 - Present
    Southern Punjab Multan, Pakistan
  • The University Of Melbourne
    Endeavour Postdoctoral Research Fellow
    The University Of Melbourne Jul 2016 - Feb 2017
    Melbourne, Victoria, Australia
    1. Assisting postgraduate students to undertake their research work2. Characterization of obg gene expression under its natural promoter3. Characterization of vlhA gene promoter for controlled expression of target genes
  • University Of Veterinary And Animal Sciences, Lahore
    Assistant Professor
    University Of Veterinary And Animal Sciences, Lahore Dec 2014 - Feb 2016
    Lahore, Pakistan
    Teaching Microbiology to under- and postgraduate studentsMolecular characterization of FMD viral isolates from PakistanMolecular characterization of HPS and IBH viral isolates from Pakistan
  • University Of Melbourne
    Doctoral Researcher
    University Of Melbourne Jun 2009 - Aug 2014
    Melbourne, Australia
    1. Worked on molecular basis of Mycoplasma synoviae pathogenesis2. Undertook comparative genomic and proteomic analysis to underpin factors involved in Mycoplasma synoviae virulence3. Conducted bioinformatic analysis of high-throughput, next generation sequencing data (SOLiD and Illumina technologies) along with Sanger sequencing data analysis4. Interpretation of Optical mapping data for scaffold building and validation of bacterial genome sequence assembly5. Transformation of Mycoplasma synoviae to characterize gene functions6. Hands on gene cloning, expression, protein purification and production of polyclonal antisera7. Development of new diagnostic tools to delineate M. synoviae vaccine strain from field isolates using Q-PCR and high-resolution melting curve analysis
  • Mahidol University, Bangkok, Thailand
    Phd Student
    Mahidol University, Bangkok, Thailand Mar 2008 - Sep 2008
    Molecular empidemiology of H5N1 avian influenza virus
  • Poultry Research Institute, Rawalplindi, Pakistan
    Veterinary Officer And Researcher
    Poultry Research Institute, Rawalplindi, Pakistan Jan 2006 - Feb 2008
    Rawalpindi, Pakistan
    Isolation and characterization of Avian influenza virus serotypes H5, H7 and H9 by Haemagglutination (HA), Haemagglutination inhibition (HI), Agar gel precipitation (AGPT), Virus neutralization tests (VN) and Polymerase chain reaction (PCR).Detection of Newcastle disease (ND), Infectious bronchitis (IB), Infectious bursal disease (IBD), Avian influenza (AI) viruses and Mycoplsma gallisepticum (MG) through Indirect Enzyme linked immunosorbent assay (ELISA)Isolation and characterization of Mycoplsma gallisepticum (MG) and Mycoplsma synoviae (MS) by cultural, morphological, biochemical, serological techniques (Growth inhibition test) and PCR from chronic respiratory disease and infectious synovitis affected poultry respectivelyPreparation of diagnostic antigen for rapid serum agglutination (RSA) test of Mycoplsma gallisepticum and Mycoplsma synoviaeCytopathological studies of different viruses on chicken fibroblast monolayerPostmortem of birds

Muhammad Akbar Shahid Skills

Molecular Biology Elisa Microbiology Pcr Qpcr Immunology Gel Electrophoresis Veterinary Polymerase Chain Reaction Cell Culture Dna Sequencing Molecular Cloning Vaccines Epidemiology Western Blotting Sequencing Protein Expression Genomics Real Time Polymerase Chain Reaction Dna Extraction Rna Isolation Veterinary Medicine Science Biotechnology Research Life Sciences Protein Purification Pathology Animal Models Immunohistochemistry Biochemistry Data Analysis Genetics

Muhammad Akbar Shahid Education Details

Frequently Asked Questions about Muhammad Akbar Shahid

What company does Muhammad Akbar Shahid work for?

Muhammad Akbar Shahid works for Bahauddin Zakariya University, Multan

What is Muhammad Akbar Shahid's role at the current company?

Muhammad Akbar Shahid's current role is Assistant Professor at Bahauddin Zakariya University, Multan.

What is Muhammad Akbar Shahid's email address?

Muhammad Akbar Shahid's email address is sh****@****.edu.au

What is Muhammad Akbar Shahid's direct phone number?

Muhammad Akbar Shahid's direct phone number is +61 3 9035*****

What schools did Muhammad Akbar Shahid attend?

Muhammad Akbar Shahid attended University Of Melbourne, University Of Agriculture, Faisalabad, University Of Agriculture, Faisalabad.

What are some of Muhammad Akbar Shahid's interests?

Muhammad Akbar Shahid has interest in Reading And Writing Research Articles, Politics, Education, Poverty Alleviation, Science And Technology, Disaster And Humanitarian Relief, Animal Welfare, Health.

What skills is Muhammad Akbar Shahid known for?

Muhammad Akbar Shahid has skills like Molecular Biology, Elisa, Microbiology, Pcr, Qpcr, Immunology, Gel Electrophoresis, Veterinary, Polymerase Chain Reaction, Cell Culture, Dna Sequencing, Molecular Cloning.

Who are Muhammad Akbar Shahid's colleagues?

Muhammad Akbar Shahid's colleagues are Abdul Basit Munir, Asia Saeed, Juweria Khawar, Sajjad Multan, Zafar Qaisar, Khurram Iftikhar, Ikram Ayoub.

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