Devon Fitzpatrick Email & Phone Number

Boston, Ma, Us

Boston, Ma, Us

Boston, Massachusetts, United States

Genome engineer• Development and testing of novel approaches for gene replacement and multiplexed editing• Analysis of gene editing outcomes through analysis of NGS, Sanger, and WGS data• Use of various gene editing modalities including Cas9, Cas12a, base editors, prime editors, and recombinases• Complex bacterial plasmid design and assembly using Gibson, Golden Gate, and restriction cloning methods• Cell culture of epithelial and endothelial elephant cells• Cell culture methods of transfection, FACS or antibiotic selection, clonal outgrowth, media preparation, and analysis in ImageJ• Determination of on- and off-target integration rates • NGS library prep for Illumina deep sequencing with in-house MiSeq• Preparation of highly efficient mRNA base editors• Gene function investigation through overexpression and RNA-Seq

Davis, California, United States

Research• Characterizing the on- and off-target mutations present in CRISPR/Cas9 gene-edited sheep through WGS and Sanger sequencing analysis• Evaluation of the growth, feed efficiency, and other phenotypes of gene-edited sheep to evaluate if they provide a production advantage• Creating an ovine genetic model for pulmonary arterial hypertension through CRISPR/Cas9 mediated heterozygous KO of the BMPR2 gene• Development of germline complementation technology in sheep through Nanos3 KO in sheep embryos followed by blastocyst complementation with donor stem cells• Wrote a substantive part of a grant proposal that was awarded $50,000• Developed skills in reproductive biology associated with embryo production, skills in genome engineering associated with genotyping embryos and animals, and skills scientific project design and statistical analysisAcademics• Mammalian Physiology of Reproduction, Gametogenesis and Fertilization, Developmental Biology and Genetics, Python, Comparative Genomics, Ethics in Biotech, Experimental Design, Public SpeakingTeaching Assistant for 6 classes• Helped teach students and led several discussions and lectures• Graded papers, tests, and presentations

• Managed the collection of ovaries, aspiration of oocytes, and shipment of oocytes to collaborators at Stanford• Mentored several new lab members in embryo production and gene editing

Davis, California

• Worked with graduate students on their projects including the disabling of pancreatic development in sheep for xenotransplantation and the generation of SOCS2 KO sheep• Produced sheep embryos via parthenogenetic activation or IVF.• Designed a gene-editing approach to creating hairy meat breeds of sheep with the goal of eliminating tail docking, improving lamb survivorship, and increasing meat yield.• Assisted with embryo transfer and estrous synchronization of ewes• Used CRISPR, electroporation, PCR, gel electrophoresis, Sanger sequencing, DNA extraction, Snap Gene, cell imaging, and bioinformatics programs• Collected semen with an artificial vagina or electroejaculator, and hand mated sheep

A highly competitive sports club that taught me valuable life lessons, the benefits of hard work, and leading by example.• 20-25 hours a week time commitment

Davis, California

Provided a safe and friendly transport experience students and residents to the UC Davis Campus. Earned Class B driver license.

Falmouth, Massachusetts, United States

• Maintained the health and wellbeing of alpacas, sheep, goats, tortoises, turkeys, chickens, and geese on a community farm. • Planted seeds, weeded, harvested and sold produce• Volunteered during the summer of 2013

Bolton, Massachusetts, United States

Trading Post Clerk in my first year, Life Guard and Merit Badge Counsellor in my second.

Ph.D., Animal Biology

Bachelors Of Science, Animal Science, Specialization: Genetics

Education record