Project 1: Preparation, characterization and evaluation of photo-activated self-transformable nanoparticles for enhanced tissue penetration for drug delivery application.Project 2: Design and optimization of protein-based immuno-nanoparticles conjugated with multivalent single domain antibodies for broad neutralization towards various SARS-CoV-2 variants.

Developed nanomaterial platforms using plant viruses for applications in biomedicine such as drug delivery carriers and imaging. Published 1 paper in Nanoscale (1st author), and submitted two in Journal of Structural Biology (1st author) and Nano Letters (3rd author).My work included producing, functionalizing and evaluating functional nanoparticles in vitro and in vivo for biomedical applications. Particularly:- Inoculated and purified plant viral nanoparticles.- Performed daily nanomaterial characterization (UV/Vis, FT-IR, FPLC, CLSM, TEM, SEM, AFM).- Conducted basic genetic manipulation (PCR, DNA cloning, protein expression).- Developed chemical and genetic methods for loading protein drugs, chemo drugs, targeting peptides, fluorescent dyes, MRI contrast agents.- Evaluated therapeutic nanoparticles in vitro and in vivo (cell culture, MTT assay, flow cytometry, tumor inoculation in mouse models, animal study, biodistribution).

Designed novel elastin-like polypeptides and investigated their self-assembling mechanisms to control their nanostructures. Published 4 papers, 3 as 1st author. - Constructed recombinant elastin-like polypeptides using DNA cloning and expressed proteins in E. coli.- Characterized nanostructures using CD spectroscopy, UV/Vis, FT-IR, AFM, TEM, SEM.