Dylan A. Howie Email & Phone Number

Valhalla, New York, United States

Valhalla, NY

Working in the Protein Biochemistry group, responsible for performing a variety biochemical and biophysical assays to characterize protein biologics to support late-stage development and commercialization efforts.

New York, NY

• Designed and optimized protocols for expressing and purifying seven different proteins, allowing for rapid and efficient production of samples for analysis via cryo-electron microscopy.
• Resolved a structure of a transmembrane protein to 9.5 angstrom resolution.
• Generated automated chromatography sequences and methods to minimize the time it takes to load samples.
• Established reputation as the team’s troubleshooter.
• Responsible for lab maintenance (maintain stocks of lab materials, troubleshooting broken equipment, cell-line maintenance, preparation of protein stocks, autoclave cycles, etc.)
• Coordinated​ ​with​ ​various​ ​lab​ ​members​ ​to​ ​ensure​ ​timely​ ​project​ ​advancement.

Ithaca, NY

• Built​ ​a​ ​numerical​ ​model​ ​to​ ​simulate​ ​the​ ​transport​ ​and​ ​capture​ ​of​ ​charged​ ​colloidal​ ​particles​ ​in​ ​depth​ ​filtration​ ​via​ ​MATLAB.
• Re-engineered​ ​an​ ​existing​ ​model​ ​for​ ​air​ ​filtration​ ​to​ ​account​ ​for​ ​factors​ ​governing​ ​aqueous​ ​particle​ ​motion​ ​(e.g.​ ​low​ ​Reynolds​ ​number fluid​ ​dynamics,​ ​Coulomb​ ​interactions,​.
• Conceived and established a method for calculating particle capture probability, paving the way for predicting filter performance.
• Acquired an extensive theoretical understanding of depth filtration processes and the key parameters that govern filter performance.
• Received​ ​the​ ​Outstanding​ ​MEng.​ ​Project​ ​Award​ ​from​ ​Cornell​ ​Chemical​ ​&​ ​Biomolecular​ ​Engineering​ ​Department.

Tarrytown, NY

• Studied​ ​the​ ​rate​ ​of​ ​foam​ ​collapse​ ​as​ ​a​ ​function​ ​of​ ​formulation​ ​composition,​ ​method​ ​of​ ​preparation,​ ​and​ ​the​ ​manner​ ​of​ ​foam​ ​production for​ ​an​ ​antifoaming​ ​product​ ​with​.
• Proposed​ ​a​ ​novel​ ​mechanism​ ​of​ ​antifoaming​ ​action​ ​by​ ​applying​ ​the​ ​results​ ​of​ ​my​ ​experiments​ ​to​ ​general​ ​antifoaming​ ​theory, which furthered Momentive's understanding of the underlying.
• Publication: Koczo, K., Howie, D., Policello, G. A., Truong, L., Brown, W. L., and Naue, J. A., “Foam Control of Trisiloxane Alkoxylate Superspreaders with Modified Trisiloxane Alkoxylates: A Mechanistic Study,”.
• Recognized on the following poster presentation by Dr. Kalman Koczo:"Mechanism of antifoams for trisiloxane alkoxylate solutions" presented at the 2016 ISAA '16 conference in San Francisco.

Baltimore, MD

• Developed/tested​ ​DNA​ ​strand​ ​displacement​ ​reaction​ ​systems​ ​via​ ​numerical​ ​simulation​ ​and​ ​subsequent​ ​experimental​ ​validation.
• Designed​ ​a​ ​numerical​ ​method​ ​in​ ​MATLAB​ ​to​ ​convert​ ​raw​ ​fluorescence​ ​data​ ​into​ ​meaningful​ ​results​ ​(concentration​ ​vs.​ ​time),​ ​which significantly​ ​increased​ ​experimental​ ​throughput​.
• Summary​ ​of​ ​techniques:​ MATLAB, DNA strand-displacement reactions, ​quantitative polymerase chain reaction (qPCR),​ ​fluorescence​ ​spectroscopy,​ polyacrylamide gel-electrophoresis (​PAGE).
• Publication:Fern, J., Scalise, D., Cangialosi, A., Howie, D., Potters, L., Schulman, R. "DNA Strand-Displacement Timer Circuits,” ACS Synth. Biol., 2017, 6 (2), pp 190-193, http://dx.doi.org/10.1021/acssynbio.6b00170.
• Recognized on the following poster presentations by Dr. Rebecca Schulman and PhD candidate Dominic Scalise: - “Continuously Active Molecular Circuits” presented at the 2015 Molecular Programming Project Conference in.

Tarrytown, NY

• Analyzed​ ​the​ ​effects​ ​of​ ​various​ ​excipients/buffers,​ ​and​ ​combinations​ ​thereof,​ ​on​ ​aqueous​ ​monoclonal​ ​antibody​ ​aggregation.
• Proposed​ ​a​ ​change​ ​to​ ​the​ ​current​ ​product​ ​formulation​ ​and​ ​backed​ ​up​ ​my​ ​claims​ ​with​ ​concrete​ ​experimental​ ​results.
• Summary​ ​of​ ​techniques:​ High-performance liquid chromatography (​HPLC),​ ​rheometer,​ ​buffer​ ​preparation,​ ​absorbance (A280).

M. Eng. Chemical Engineering

Activities and Societies: GPA: 3.75. Awarded 2016 Outstanding M. Eng Research Project Award. Cornell Honor Society. Biotechnology Club.

Bs Chemical & Biomolecular Engineering

Activities and Societies: American Institute of Chemical Engineers / Society for Biological Engineers; Engineers Without Borders; Men’s.