Dylan A. Howie

Dylan A. Howie Email and Phone Number

Research Associate at Regeneron Pharmaceuticals, Inc. @ Regeneron
tarrytown, new york, united states
Dylan A. Howie's Location
Somers, New York, United States, United States
Dylan A. Howie's Contact Details

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About Dylan A. Howie

Biochemical engineer interested in forwarding therapeutic capability by optimizing bioprocesses. Possess extensive practical experience expressing, purifying, and characterizing proteins.

Dylan A. Howie's Current Company Details
Regeneron

Regeneron

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Research Associate at Regeneron Pharmaceuticals, Inc.
tarrytown, new york, united states
Website:
regeneron.com
Employees:
9030
Dylan A. Howie Work Experience Details
  • Regeneron
    R&D Specialist
    Regeneron Nov 2021 - Present
    Valhalla, New York, United States
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  • Regeneron Pharmaceuticals, Inc.
    Research Associate
    Regeneron Pharmaceuticals, Inc. Sep 2018 - Oct 2021
    Valhalla, Ny
    Working in the Protein Biochemistry group, responsible for performing a variety biochemical and biophysical assays to characterize protein biologics to support late-stage development and commercialization efforts.
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  • Memorial Sloan Kettering Cancer Center
    Structural Biology Research Technician
    Memorial Sloan Kettering Cancer Center Nov 2016 - Sep 2018
    New York, Ny
    ● Designed and optimized protocols for expressing and purifying seven different proteins, allowing for rapid and efficient production of samples for analysis via cryo-electron microscopy.● Resolved a structure of a transmembrane protein to 9.5 angstrom resolution.● Generated automated chromatography sequences and methods to minimize the time it takes to load samples.● Established reputation as the team’s troubleshooter.● Responsible for lab maintenance (maintain stocks of lab materials, troubleshooting broken equipment, cell-line maintenance, preparation of protein stocks, autoclave cycles, etc.) ● Coordinated​ ​with​ ​various​ ​lab​ ​members​ ​to​ ​ensure​ ​timely​ ​project​ ​advancement. ● Summary​ ​of​ ​techniques:​ Size-exclusion chromatography (SEC) (AKTA), fluorescence size-exclusion chromatography (FSEC), affinity chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), cell culture (bacteria, insect, mammalian), absorbance (A280), viral infection (baculovirus), molecular cloning, polymerase chain reaction (PCR), quantitative polymerase chain reaction (qPCR), DNA transfection/transformation, miniprep/maxiprep, dialysis.
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  • School Of Chemical & Biomolecular Engineering, Cornell University
    Graduate Researcher
    School Of Chemical & Biomolecular Engineering, Cornell University Sep 2015 - May 2016
    Ithaca, Ny
    ● Built​ ​a​ ​numerical​ ​model​ ​to​ ​simulate​ ​the​ ​transport​ ​and​ ​capture​ ​of​ ​charged​ ​colloidal​ ​particles​ ​in​ ​depth​ ​filtration​ ​via​ ​MATLAB. ● Re-engineered​ ​an​ ​existing​ ​model​ ​for​ ​air​ ​filtration​ ​to​ ​account​ ​for​ ​factors​ ​governing​ ​aqueous​ ​particle​ ​motion​ ​(e.g.​ ​low​ ​Reynolds​ ​number fluid​ ​dynamics,​ ​Coulomb​ ​interactions,​ ​lubrication​ ​forces,​ ​Brownian​ ​motion,​ ​membrane​ ​fouling)​ ​to​ ​calculate​ ​particle​ ​trajectories. ● Conceived and established a method for calculating particle capture probability, paving the way for predicting filter performance.● Acquired an extensive theoretical understanding of depth filtration processes and the key parameters that govern filter performance.● Received​ ​the​ ​Outstanding​ ​MEng.​ ​Project​ ​Award​ ​from​ ​Cornell​ ​Chemical​ ​&​ ​Biomolecular​ ​Engineering​ ​Department.
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  • Momentive Performance Materials, Inc.
    Specialty Fluids Research & Development Intern
    Momentive Performance Materials, Inc. May 2015 - Aug 2015
    Tarrytown, Ny
    ● Studied​ ​the​ ​rate​ ​of​ ​foam​ ​collapse​ ​as​ ​a​ ​function​ ​of​ ​formulation​ ​composition,​ ​method​ ​of​ ​preparation,​ ​and​ ​the​ ​manner​ ​of​ ​foam​ ​production for​ ​an​ ​antifoaming​ ​product​ ​with​ ​previously​ ​undocumented​ ​properties. ● Proposed​ ​a​ ​novel​ ​mechanism​ ​of​ ​antifoaming​ ​action​ ​by​ ​applying​ ​the​ ​results​ ​of​ ​my​ ​experiments​ ​to​ ​general​ ​antifoaming​ ​theory, which furthered Momentive's understanding of the underlying mechanisms governing the behavior of this particular product.● Publication: Koczo, K., Howie, D., Policello, G. A., Truong, L., Brown, W. L., and Naue, J. A., “Foam Control of Trisiloxane Alkoxylate Superspreaders with Modified Trisiloxane Alkoxylates: A Mechanistic Study,” Pesticide Formulation and Delivery Systems: 37th Volume, Formulations with Ingredients on the EPA’s List of Minimal Concern, ASTM STP1602, R. K. Edlund, Ed., ASTM International, West Conshohocken, PA, 2018, pp. 63–77, http://dx.doi.org/10.1520/ STP1602201601352.● Recognized on the following poster presentation by Dr. Kalman Koczo:"Mechanism of antifoams for trisiloxane alkoxylate solutions" presented at the 2016 ISAA '16 conference in San Francisco.
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  • Schulman Dna Nanotechnology & Intelligent Materials Group Lab, Johns Hopkins University
    Research Lab Assistant
    Schulman Dna Nanotechnology & Intelligent Materials Group Lab, Johns Hopkins University Sep 2013 - May 2015
    Baltimore, Md
    ● Developed/tested​ ​DNA​ ​strand​ ​displacement​ ​reaction​ ​systems​ ​via​ ​numerical​ ​simulation​ ​and​ ​subsequent​ ​experimental​ ​validation. ● Designed​ ​a​ ​numerical​ ​method​ ​in​ ​MATLAB​ ​to​ ​convert​ ​raw​ ​fluorescence​ ​data​ ​into​ ​meaningful​ ​results​ ​(concentration​ ​vs.​ ​time),​ ​which significantly​ ​increased​ ​experimental​ ​throughput​ ​and​ ​overall​ ​efficiency,​ ​allowing​ ​for​ ​quicker​ ​results.● Summary​ ​of​ ​techniques:​ MATLAB, DNA strand-displacement reactions, ​quantitative polymerase chain reaction (qPCR),​ ​fluorescence​ ​spectroscopy,​ polyacrylamide gel-electrophoresis (​PAGE).● Publication:Fern, J., Scalise, D., Cangialosi, A., Howie, D., Potters, L., Schulman, R. "DNA Strand-Displacement Timer Circuits,” ACS Synth. Biol., 2017, 6 (2), pp 190-193, http://dx.doi.org/10.1021/acssynbio.6b00170.● Recognized on the following poster presentations by Dr. Rebecca Schulman and PhD candidate Dominic Scalise: - “Continuously Active Molecular Circuits” presented at the 2015 Molecular Programming Project Conference in San Francisco. - “Sustained Enzyme Free Molecular Circuits” presented at the 2015 Biophysical Society Conference in Baltimore. - “Synthetic DNA Delay Circuits for Temporal Coordination” presented at the 2015 SEED Conference in Boston.
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  • Regeneron Pharmaceuticals
    Therapeutic Protein Formulations Intern
    Regeneron Pharmaceuticals May 2013 - Aug 2013
    Tarrytown, Ny
    ● Analyzed​ ​the​ ​effects​ ​of​ ​various​ ​excipients/buffers,​ ​and​ ​combinations​ ​thereof,​ ​on​ ​aqueous​ ​monoclonal​ ​antibody​ ​aggregation. ● Proposed​ ​a​ ​change​ ​to​ ​the​ ​current​ ​product​ ​formulation​ ​and​ ​backed​ ​up​ ​my​ ​claims​ ​with​ ​concrete​ ​experimental​ ​results. ● Summary​ ​of​ ​techniques:​ High-performance liquid chromatography (​HPLC),​ ​rheometer,​ ​buffer​ ​preparation,​ ​absorbance (A280).
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Dylan A. Howie Skills

Chemical Engineering Research Chemistry Cell Biology Matlab Data Analysis Science Laboratory Skills Dna Laboratory Microsoft Excel Cell Culture Protein Purification Polymerase Chain Reaction High Performance Liquid Chromatography Akta Transient Transfection Molecular Cloning Baculovirus Miniprep Lab Managment Sds Page Western Blotting Size Exclusion Unicorn Empower Dnastar Elisa Affinity Chromatography Numerical Simulation Agarose Gel Electrophoresis Site Directed Mutagenesis Subcloning Troubleshooting Pipetting Graphpad Prism Minitab Relion Image J Microsoft Powerpoint Microscopy Microsoft Word Microsoft Office

Dylan A. Howie Education Details

Frequently Asked Questions about Dylan A. Howie

What company does Dylan A. Howie work for?

Dylan A. Howie works for Regeneron

What is Dylan A. Howie's role at the current company?

Dylan A. Howie's current role is Research Associate at Regeneron Pharmaceuticals, Inc..

What is Dylan A. Howie's email address?

Dylan A. Howie's email address is dh****@****ell.edu

What is Dylan A. Howie's direct phone number?

Dylan A. Howie's direct phone number is +191451*****

What schools did Dylan A. Howie attend?

Dylan A. Howie attended Cornell University, The Johns Hopkins University.

What are some of Dylan A. Howie's interests?

Dylan A. Howie has interest in Chemical Engineering, Skiing, Soccer, Fitness, Music.

What skills is Dylan A. Howie known for?

Dylan A. Howie has skills like Chemical Engineering, Research, Chemistry, Cell Biology, Matlab, Data Analysis, Science, Laboratory Skills, Dna, Laboratory, Microsoft Excel, Cell Culture.

Who are Dylan A. Howie's colleagues?

Dylan A. Howie's colleagues are Yonina Rosenbaum, Steve Lenzinger, Kia Manoochehri, Jaime Rodriguez, Shira Adler, Kathleen Dingee, Theresa Silsby.

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