Dylan B. Email & Phone Number

Remote

Dallas, Texas, United States

Reston, Virginia, United States

Austin, Texas, United States

College Station, Texas

• Developed and implemented multiple ELISA based and cell based assays per client specification to test drug efficacy/potency.• Streamlined workflows to establish a more high-throughput work environment.• Trained and worked alongside DoD/military personnel to implement rapid testing of Coronavirus vaccine candidate lots.

San Diego, California

• Development of assays to identify peptide sequences that trigger an immune response to proteins of interest to assist future drug development projects.• Isolated and sequenced DNA from patient samples to identify alleles of interest, as well as purification of patient antibodies for usage in assays.• Worked with and cultured cells isolated from patient samples for usage in cell based assays.• Trained and lead a group of 6 scientists in their duties, as well as performed regular performance reviews and refreshers for skills.

San Diego, California

Designed and developed cell based assays for small molecule and protein drug candidates, including establishing dose-responses for the various therapeutic candidates, as well as identification of pertinent targets for future studies.Created and maintained various cell lines for usage in cell based assays.Isolated and purified RNA for use in qPCR as well as protein for use in Western Blot to establish knock-out of target genes in transgenic animals.Analyzed data and produced presentations to update group with progress on various projects.

Santa Clara, California

Analyzed proteins and reagents via HPLC (SEC, F-SEC, Reversed Phase and IEX), Circular Dichroism, DLS, UNCLE, Osmolality, Refractive Index, Conductivity, and fluorescence/absorbance via NanoDrop.Developed assays and SOPs for analysis of membrane protein complexes using HPLC, CD, DLS, and UNCLE following GLP standards.Used GLP standards to produce reagents and proteins (via Akta FPLC) for the development of nanopore sequencer.Generated and produced batch records for all consumables, including QC in- formation of all reagents produced.Developed extensive QC experience for proteins, lipids, and buffers.

Riverside, California

Development of lesson plans, power-points, curriculum, tests, quizzes and labs to facilitate learning of complex scientific material using pedagogical techniques. Utilization of communication techniques to break down complex scientific concepts into simpler terms, facilitating learning and mastery of the subject matter for students of all knowledge levels.

Loma Linda, California

Worked with melanoma cancer cells to identify specific cell surface markers indicative of a tumor initiating cell, specifically working to isolate and culture different subpopulations of the melanoma cancer cells using FACS and Flow Cytometry. Performed various molecular biology techniques to purify proteins, as well as to ascertain fold-changes in RNA expression within melanoma cancer cells.

Loma Linda, California

Developed and wrote protocols for research pertaining to cellular senescence and its effect on SK/IK channel activity within the cerebrovascular endothelium, as well as the impact of Amyloid beta on these same channels. This included isolation of middle cerebral arteries, solution production, and production of various pharmacological agents at varying concentrations. Utilized various molecular biology techniques including western blotting and PCR to ascertain gene expression and protein expression changes from transgenic animals when compared to baseline.

Loma Linda, California

Established standard protocols for isolation and cannulation of middle cerebral arteries, in addition to training other researchers in microdissection and cannulation techniques. Recorded and interpreted data from myography experiments. Coordinated a streamlined workflow to share data between labs working on the grant. Produced an excel spreadsheet that would calculate pertinent data from each experiment, and output the data to a unified data sheet. Trained post-Doctoral researcher in the technique to take over the Myography Core after I began graduate school.