Dylan Perera Email & Phone Number

Vancouver, BC, CA

Vancouver, British Columbia, Canada

My MSc project aims to develop Nicotiana benthamiana (a tobacco relative) as an industrial-scale production system for the anti-diabetic compound Montbretin A (MbA). MbA production was previously achieved in N. benthamiana by Agrobacterium-mediated infiltration of montbretia genes. However, it is necessary to greatly increase MbA yields to meet anticipated.

Leiden, South Holland, Netherlands

I visited the Irmisch lab as part of my MSc research in the Bohlmann lab. During my time in Leiden, I primarily focused on addressing the question of side-product formation in MbA-bioengineered N. benthamiana. To this end, I applied bioinformatic, metabolomic, and in vitro assay approaches to identify undesired side-products and to identify and.

Paris, Île-de-France, France

iGEM is a worldwide engineering biology competition for student-led teams that apply molecular biology, engineering approaches, and social context to important world issues.Following 3 years of competing in iGEM as a student, I returned to serve as a judge for the 2021 and 2022 iGEM competitions.I thoroughly reviewed teams’ documentation and presentations.

Kitchener, Ontario, Canada

Used metabolic engineering strategies to develop E. coli and Corynebacterium glutamicum as production systems for sustainable textile dyesUsed Gibson assembly to build an E. coli-C. glutamicum shuttle vector encoding dye biosynthesisTroubleshot C. glutamicum transformation using various heat shock and electroporation protocols Optimized fermentation.

Used metabolic engineering to increase production of industrially-relevant isoprenoids in E. coliEmployed CRISPR tools to insert variations of a synthetic isopentenol-utilisation pathway into the E. coli chromosome for reduced metabolic burden and stable maintenanceDesigned CRISPR insertion experiments using online tools and used Gibson assembly to build.

Waterloo, Ontario, Canada

iGEM is a worldwide engineering biology competition for student-led teams that apply molecular biology, engineering approaches, and social context to important world issuesThe 2020 project seeks to use metal-binding proteins to recover valuable metals from industrial wastes: Proteins will be identified, engineered to improve specificity and affinity, and.

Waterloo, ON

iGEM is a worldwide engineering biology competition for student-led teams that apply molecular biology, engineering approaches, and social context to important world issuesTeam earned a silver medal for 2019 project, which sought to confer linuron resistance to the root-nodule forming bacterium B. diazoefficiens by expressing a two-step linuron degradation.

Toronto, Ontario, Canada

Used bioinformatic approaches to investigate the prevalence and identity of reductive dehalogenases in enrichment culture metagenomes, focusing on hexachlorocyclohexane (HCH) dechlorinating enzymesQuality control, assembly, and annotation of metagenome sequencing data were performed at the command line using MetaErg and with the online workflow manager.

Vancouver, British Columbia, Canada

Tasked with studying microbial selenate reduction using molecular biology and biochemistry approaches in an engineering-focused research group Independently developed and validated methods for extracting genomic DNA from difficult environmental and industrial samples including biofilmsResponsible for maintenance and sampling of a delicate anaerobic.

Waterloo, ON

Involved in development of fluorescent lateral flow immunoassays for quantification of allergy-related antibodies in patient blood samples, managing rapidly changing start-up research prioritiesIndependently optimised preparation and analysis of antibody-microparticle conjugates and biotinylated allergen components for use in assay research and.

Guelph, ON

Nominated by employer for university-wide “Co-op Student of the Year” awardIndependently designed and performed experiments based on existing literature and changing start-up research priorities with minimal supervisionCompleted multi-step organic reactions to produce polysaccharide nanomaterial therapeuticsCharacterised resulting nanomaterials by.

Waterloo, ON

Investigated ion exchange chromatography for the downstream purification of monoclonal antibodies, optimising process conditions to improve yield and reduce aggregationIndependently designed and performed experiments to evaluate the use of thioflavin T as an extrinsic fluorophore for quantifying and characterising antibody aggregates Employed analytical.

St. Catharines, ON

Demonstrated resourcefulness by following protocols for syntheses of long-chain dicarboxylic acids as a high school student with little assistance or previous knowledge Improved understanding of organic synthesis techniques, laboratory safety, and analytical methods including 1H NMR spectroscopyPresented work at regional science fair and won several.

Master Of Science - Msc, Genome Science & Technology

Bachelor Of Science - Bsc, Honours Biochemistry

Associate Piano Performer Diploma (Accm), Piano Performance

Prepared and performed advanced-level piano pieces and completed several written exams in music theory and history, achieving the highest.

High School