Eashwar I. Email & Phone Number

San Ramon, CA, US

Emeryville, California, US

• Protein purification of recombinant protein with AKTA FPLC: Affinity/Ion Exchange/Mixed Mode/Size Exclusion Chromatography
• Protein characterization assays for purity/homogeneity
• Measure and Improve Enzyme performance for multiple proprietary Enzymes with: plate reader based and fragment analysis based kinetic assays.
• Proficient with Automated Liquid Handling Machines
• Designed, Onboarded, and Troubleshooted various QC assays for performance, purity, and stability of two proprietary enzymes
• Independently plan and execute experiments

Leverkusen, North Rhine-Westphalia, DE

• Work closely under Downstream Production Team to improve process for monoclonal antibody purification
• Handle and troubleshoot Industry standard protein purification technology such as: Akta, Akta Ready, and Akta Plus as part of downstream processing for mAB.
• Understand and clearly communicate complexity in terms of documentation, samplehandling, automation and cGMPs etc. for different phases of mAB product life cycle.
• Understand the purpose of IOPQ activities, including commissioning of new equipment,execution of protocols, and what passing qualification means in order to ensure FDA compliance;

Santa Barbara, CA, US

Participated in 10 week long rigorous workshop focused on the foundations of Bioinformatics. Workshop focused on building proficiency in Command Line, BLAST, and other programs in order to analyze Biological Data including: Gene sequences, Protein sequences, & Evolutionary Relationships

Santa Barbara, CA, US

Worked on a project exploring how conformational changes are associated with activity of various E.coli proteins involved in chemotaxis. Proteins of interest were CheY and Tar receptor proteins. My main role in the project was to purify proteins from cell lysate through Ni-NTA chromatography, and Size Exclusion Chromatography. Techniques Involved.

Bachelor Of Science - Bs, Pharmacology

Education record