Cambridge Primary Science Teacher - Year 6Cambridge Lower Secondary Teacher - Year 7IGCSE Environmental Management Teacher - Years 10 & 11

Cambridge Primary Science - Year 6Cambridge Lower Secondary Science - Years 7 & 8

Integrated Sciences Teacher (grade 6, 7 and 8)MYP Personal Project Supervisor

Biology teacher - grade 9 and 10Maths teacher - grade 8, 9 and 10

Research related with autophagy, apoptosis, mainly how caspase pathway was activated when cells where under starvation.Main techniques used:- Gradient SDS-Page - Western Blot- Protein lysates from cell cultures- Protein quantification- FACS- Cell Culture (A549 and U1810 cell lines)- Caspase activity analysis

Identification and validation of new potential targets for neuroprotection following experimental demyelination.Main tchniques used:- Protein lysates- SDS-Page gel- Silver staining- Proteomic techniques (iTRAQ)- Western Blot- Immunohistochemistry- Cryostat cuts- Statistical analysis- Data quantification- Spinal cord dissection- Fluorescent microscopy

Research related with RhoGTPases. Rho-mediated signaling of myelin-associated neurite outgrowth inhibitors: a role for rhob/c.Techniques used:- Cell culture of various cell lines (HeLa, PC12, primed PC12 neuronal-like, MSC80 schwannoma, NIH 3T3 fibroblasts)- Primary cell culture (mouse/rat) Shwann cells- Lentivirus production- shRNA- Lentivirus transfection- Fluorescent microscopy and imaging- Immunocytochemistry- DNA/RNA extraction- Bioinformatic… Show more Research related with RhoGTPases. Rho-mediated signaling of myelin-associated neurite outgrowth inhibitors: a role for rhob/c.Techniques used:- Cell culture of various cell lines (HeLa, PC12, primed PC12 neuronal-like, MSC80 schwannoma, NIH 3T3 fibroblasts)- Primary cell culture (mouse/rat) Shwann cells- Lentivirus production- shRNA- Lentivirus transfection- Fluorescent microscopy and imaging- Immunocytochemistry- DNA/RNA extraction- Bioinformatic tools (online tools for alignment, used to choose primers) Show less

State company for quality control of fisheriesMolluscs and water analysis for quality control of fisheriesMain activities and responsibilities:- Quality analysis of the coastal bivalve molluscs.Techniques used- Microbiologic cultures (solid and semi-solid)- Microorganisms counting,- Extraction of the mulluscs content.