Eduardo Barrranco Email & Phone Number

San Diego, California, United States

San Diego, California, United States

• Independently develop and run Multicolor Intracellular Staining and collect data doing Flow Cytometry using the BD LSRFortessa Instrument. • B-cell staining for COVID and MERS spike protein. • Experimental analysis using FLowJo software and Prism software. Data and graphs were then used to present at department meetings.• Troubleshoot problems arising from assays and identify the next steps with the project lead.• Managing and ordering antibody stock for experiments.• Maintain GLP/GCLP compliance and organize appropriate documentation• Organize and update Lab Notebook.

United States

• Design and carry out ELISA experiments using human sera from clinical trials. • Assisted with Optimization, Qualification, and Validation of an ELISA for clinical trial experiments.• Design and carry out ELIspot experiments using PBMCs from patients post-vaccination to support clinical trials. • Perform Cytokine Release assays using Biolegends Legendplex and collect data by doing Flow Cytometry using the BD FACSDiva software.• Carry out Extended Stimulation experiments for expanding CD4+ and CD8+ T-cells specific to a pathogen of interest.• PBMC Isolation/Blood Processing of incoming human samples from various timepoints and clinical sites. • Receive and organize clinical trial samples in LN2 tanks and refrigerators to appropriate locations.• Use the FreezerPro software to organize samples for identification.• Help write SOPs to be used for clinical trial experiments. • Perform general laboratory duties such as reagent prep, monthly tasks, ordering supplies for the laboratory, and organizing the laboratory room.• Knowledgeable in GCLP and BSL-2 requirements.

San Diego, California, United States

Bachelor Of Science - Bs, Microbiology, General

High School Diploma