Remote contractor for Navigate BioPharma / Novartis. Perform gating, analyses and scientific review of high dimensional flow cytometry data collected from immuno-oncology clinical trials. Utilize technical and scientific expertise in immunophenotyping to interpret and troubleshoot data. Analysis of high dimensional (12+ color) flow cytometry data, specifically with T-cell functional subsets (memory, activation, exhaustion) and/or Myeloid cell phenotyping (MDSC, pDC etc). FlowJo software.

Immunotherapy process development, manufacturing of CAR T cells for the treatment of hematologic malignancies, development and optimization of multi-color flow cytometry panels, cell culture (cancer cells and immune cells), T cells activation, stimulation, viral transduction, expansion, detailed documentation, preparation of protocols, SOPs, study reports, batch records, writing documents, presentation of the results at meetings, drafting for FDA regulatory submissions, knowledge of GLP/GMP… Show more Immunotherapy process development, manufacturing of CAR T cells for the treatment of hematologic malignancies, development and optimization of multi-color flow cytometry panels, cell culture (cancer cells and immune cells), T cells activation, stimulation, viral transduction, expansion, detailed documentation, preparation of protocols, SOPs, study reports, batch records, writing documents, presentation of the results at meetings, drafting for FDA regulatory submissions, knowledge of GLP/GMP regulations, technology transfer, data plotting, and statistical analyses, data verification, methods validation, quality control, critical-thinking, problem-solving. Show less

Pancreatic cancer immunology investigation, syngeneic and GEMM models, analysis of TILs and immune TME, independent project management: experiment design, performing experiments, data analytics, statistical analyses, interpretation of experiments, assay troubleshooting, results presentation. High-dimensional flow cytometry, tumor immunophenotyping, spatial analysis of immune cells in tumor sections using machine learning InForm software, cell culture, bioassays, molecular biology assays… Show more Pancreatic cancer immunology investigation, syngeneic and GEMM models, analysis of TILs and immune TME, independent project management: experiment design, performing experiments, data analytics, statistical analyses, interpretation of experiments, assay troubleshooting, results presentation. High-dimensional flow cytometry, tumor immunophenotyping, spatial analysis of immune cells in tumor sections using machine learning InForm software, cell culture, bioassays, molecular biology assays, cell-based assays (cytotoxicity, cytokine secretion, ELISA, MTT, apoptosis with Annexin V or Propidium iodide, cell cycle, proliferation and cell count with trypan blue), gene modification (transfection, transduction, lentivirus vectors, viral titer), dd/qPCR, grants & manuscripts writing, management of multiple projects, training and instructing personnel in flow cytometry, mentoring students on the topics: immunology, pancreatic cancer, tumor microenvironment. Co-authored two publications, including one as the first author. Show less

Investigated DNA mismatch repair and chromatin at the Biochemistry and Molecular Biology Department of SIU School of Medicine. Co-authored three publications. Skills: virus culture, molecular cloning, transformation, transfection, transduction, protein tagging, protein over-expression, protein purification, chromatography, HPLC, Unicorn Control software, spectroscopy, colony-forming assay, Western Blot, Southern Blot, Far Western Blot, Immunoprecipitation, cell culture techniques (Human and… Show more Investigated DNA mismatch repair and chromatin at the Biochemistry and Molecular Biology Department of SIU School of Medicine. Co-authored three publications. Skills: virus culture, molecular cloning, transformation, transfection, transduction, protein tagging, protein over-expression, protein purification, chromatography, HPLC, Unicorn Control software, spectroscopy, colony-forming assay, Western Blot, Southern Blot, Far Western Blot, Immunoprecipitation, cell culture techniques (Human and Insect cells), shRNA knockdown, in vitro multi-component biochemical assays, nucleosome assembly, PCR, DNA extraction, data analysis, experimental design, Adobe Illustrator, Adobe Photoshop, ImageJ, ImageQuant, DNAStrider, Serial Cloner. Review scientific literature, design and execute experiments, quantify and analyze data, and present results, introduce and optimize a new technique in the lab, train and supervise graduate students. Show less

Preparation of plasmid DNA/virus positive control for PCR test systems, manufacturing of molecular diagnostic systems for detection of over 100 infectious diseases and genetic disorders, stability studies, quality control of the product, SOP writing, supervising a team of four technologists and performing qPCR, RT-PCR, DNA/RNA isolation.

Investigated regulation of gene expression using fish embryo model. Performed genomic DNA isolation, PCR, fluorescent microscopy, hormone injections into fish, in vitro fertilization, electroporation, microinjections into fish embryos, histochemistry, co-authored two publications. Research was a part of the Engineering degree project.