Eli Fine, Ph.D Email & Phone Number

Smyrna, Ga, Us

Philadelphia, Pennsylvania, Us

● Architecting and leading the technical implementation of a robust software stack enabling execution of scientific workflows on a cutting-edge custom-built 3D cell culture automation workcell● Partnering with scientists to gather requirements, prioritize feature development, and maintain a dynamic Agile backlog, ensuring alignment with R&D and client goals● Developing high-performance device drivers for complex instruments such as Zeiss LSM Confocal, Hamilton Zeus pipettes, KUKA robotic arm, PhoenixContact PLC, and Hiwin motion stage, enabling coordinated asynchronous operations. Utilizing tooling such as Python, C#, gRPC, CAN bus, and TCP sockets● Designing a virtualized state representation for all devices and objects in the workcell, facilitating high-fidelity in silico simulations and ensuring robust Continuous Integration testing● Built and deployed a cloud-first scalable architecture for fleet management of multiple workcells; containerized deployment managed by AWS Systems Manager for code executing on-prem, which communicates with a purpose-built high availability full stack web application

Providing experimental design, computational analysis, operational efficiency improvements, patent filings, and grant writing services to academic institutions and life science companies ranging from angel/venture/SBIR funded start-ups to Fortune 500. See http://www.EliFine.com for more information.▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬● Designing CRISPR and TALEN nucleases for optimal on-target activity and low off-target activity● Designing donor plasmids to facilitate therapeutic gene repair● Viral integration site analysis● Creating drawings and supporting information for patent claims● Subject Matter Expert analyses for Venture Capital Due Diligence

San Diego, Ca, Us

● Leading an Agile team of robotic and software engineers to architect and implement robotic laboratory solutions across the company (five sites across CA, FL, MA)● Projects involving automated liquid handlers, robotic arms and peripherals, creating AWS cloud data analysis workflows, and interacting other cloud LIMS/ELNs● Biological focus areas include genome engineering (human and E. coli), cell line development, synthetic biology, analytical assays, cell-free protein synthesis, and downstream purification

Seattle, Washington, Us

● Leading a full-stack Agile software team to develop desktop software controlling novel scientificinstrumentation, and customer cloud analysis portals to perform large scale analyses on collected data.● Designing laboratory automation solutions to accelerate internal R&D and sell to external customers● PI for NIH SBIR grant to investigate manufacturability of cryopreserved Assay Ready Plates of iPSC-CardioMyocytes (1R43HL154928-01)● Subaward PI conducting AI analysis on data collected by HESI consortium for next gen drug safety screening

Served as the primary architect and manager of Coyne Scientific's highly automated stem cell laboratory and data analysis pipelines to study the effects of genetic variation on in vitro toxicity responses of human cells to pharmaceutical compounds. ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬SR. DIRECTOR OF DATA SCIENCE & LABORATORY AUTOMATION (January 2017 - Present)● Communicated scientific results to clients, executives, and investors; presented at national conferences● Designed, integrated and programmed a $1 million automated system for human stem cell culture & assays● Populated and analyzed a large scale database of internal results containing tens of millions of data rows● Managed a small software team using Agile to build our research tools infrastructure & ensure code quality● Developed a model describing population variance of adverse effects in vitro applying significance testing ● Created a digital biological image data analysis pipeline to transform raw data into actionable results● Optimized performance of custom data analysis pipelines using multiprocessing and cloud computingDIRECTOR OF GENOMIC SCIENCE (July 2015 - December 2017)● Developed an assay from bench through analysis for RASL-seq whole transcriptome profiling of human cells● Constructed and administered a MySQL database to allow querying of in-house scientific & operational data● Interviewed and hired staff at levels from technician through PhD as the scientific team grew from 2 to 7● Automated data visualization using matplotlib and LaTeX to create reports for clients and for internal use

Atlanta, Georgia , Us

Optimized engineered nucleases for genetic correction of sickle-cell anemia▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬● Constructed TAL Effector Nucleases and CRISPR/Cas9 systems● Constructed donor repair templates● Optimized DNA repair pathway choice● Discerned factors affecting nuclease activity and creating models to predict optimal target sites● Analyzed nuclease off-target effects and creating bioinformatics models to predict off-target sites

● Developed a laboratory protocol for retroviral integration site analysis and standardized it as an SOP● Created a custom bioinformatics pipeline to process NGS deep sequencing data for integration site analysis

Providence, Rhode Island, Us

Used bioinformatic and molecular biology techniques to find evidence for novel cases of stop codon readthrough in Drosophila and human genomes.

Phd, Biomedical Engineering

Management Of Technology Certificate

B.S., Biomedical Engineering