Elisabeth (Lisa) Hansen Email and Phone Number

• Develop, optimize and perform in vitro assays using cells isolated from normal human blood. • Evaluation of mode of action mechanisms and testing of new drugs for Inflammation, and autoimmune diseases• Purification of different cell types such as B, T, NK and monocytes from human blood using AutoMACS / EasySep and RosetteSep. • MLR (mixed lymphocyte culture) • Differentiation of primary cells and monocytes to dendrite cells.• Stimulation assays (aCD3, aCD2, cytokine)… Show more • Develop, optimize and perform in vitro assays using cells isolated from normal human blood. • Evaluation of mode of action mechanisms and testing of new drugs for Inflammation, and autoimmune diseases• Purification of different cell types such as B, T, NK and monocytes from human blood using AutoMACS / EasySep and RosetteSep. • MLR (mixed lymphocyte culture) • Differentiation of primary cells and monocytes to dendrite cells.• Stimulation assays (aCD3, aCD2, cytokine), with blood and various cell types.• Measurement of cell activation, proliferation, cytokine release using ELISA/Bioplex/FACS /Western blotting.• Optimization of method for purification of lymphocytes subset from whole blood (MACS purification system) for use in identification of biomarker for clinic trials.•Super user and responsible For LSRII, Flow Cytometry (multicolor). Show less

• Optimization of murine functional assays with primary cells(e.g. bone degradation (RANKL)-TRAP assay) and implementation of B-and T cell assays used for investigation of autoimmune diseases such as Osteoporosis, RA and Alzheimer.• Development of murine T cell lines and B-and T cell hybridomas.• Lymphocyte activation –MLR (mixed lymphocyte culture).• Transfection of murine cell lines.• Implementation of ELISA, for use in the clinic (titer determination).• Transient and… Show more • Optimization of murine functional assays with primary cells(e.g. bone degradation (RANKL)-TRAP assay) and implementation of B-and T cell assays used for investigation of autoimmune diseases such as Osteoporosis, RA and Alzheimer.• Development of murine T cell lines and B-and T cell hybridomas.• Lymphocyte activation –MLR (mixed lymphocyte culture).• Transfection of murine cell lines.• Implementation of ELISA, for use in the clinic (titer determination).• Transient and stable transfection of insect cells(S2-cells)• T-cell activation using Elispot.• Responsible for Cell facilities and Cell banking Show less