Studying the metabolic regulation of Stem Cell activity in vivo using novel genetic reporters

Assistant Management

Purification and crystallization of organic compounds. Synthesis and characterization of biologically active molecule. Reactions of peptides modification. Cell proliferation assay. Biological activity of compounds assay. Flow cytometry analysis.

Basic techniques of cellular and molecular biology. Isolation of peripheral blood lymphocytes. Knowledge how to safely work with cell cultures. Cell lines maintenance, cell counting, setting up a grown curve. Freezing and thawing cells. Isolation, maintenance in culture and characterization of hematopoietic stem cells and mesenchymal stem cells from tissues. Extract DNA from tissues /cellules. Amplification and analysis of DNA. Make an enzymatic amplification of DNA. Perform easy basic… Show more Basic techniques of cellular and molecular biology. Isolation of peripheral blood lymphocytes. Knowledge how to safely work with cell cultures. Cell lines maintenance, cell counting, setting up a grown curve. Freezing and thawing cells. Isolation, maintenance in culture and characterization of hematopoietic stem cells and mesenchymal stem cells from tissues. Extract DNA from tissues /cellules. Amplification and analysis of DNA. Make an enzymatic amplification of DNA. Perform easy basic manipulations for cloning and DNA analysis. Analyze DNA by electrophoresis. Chimerism testing analysis after transplant. Show less