Elise Fleury Email & Phone Number

Plan-Les-Ouates, Ge, Ch

Genève, Suisse

Genève, Suisse

Région De Genève, Suisse

Area : Cell Biology, Immunology.Tasks: Assess the manufacturability of the antibody candidates(bispecific) and product batches: (i) Generate stable cell pools, (ii) Select cell pools by flow cytometry; (iii) IgG quantification, (iv) Antibody purification and quality control of the delivered batch.Development of new methods to improve the process (establishment of a transient transfection protocol, cells surface marker study).Antibodies functional testing after process improvement step.Support Pharmacology team for antibodies functional testing, when it's needed.Scientific skills:Scientific watch.Experiment Scheduling, experiments.GLP respect.Work in ultra-clean area.Results analysis and interpretation.Draft and daily use QA documentation.Written and oral result presentation. (English/French)Reagent and lab stock management.Equipment management.Technical skillsCell biology:Cell culture (CHO, PBMC), fed-batch culture, cells transfections by electroporation or chemical method, blood cells isolation, cell-based assays (ADCC, ADCP). Immunology:Flow cytometry, IgG quantification by Octet (bioLayer interferometry).Molecular biology:DNA extraction (after bacteria culture), protein separation by Agilent Bioanalyser.Biochemistry:Antibody purification (AKTA Prime)Analytic:Isoelectric Focusing (IEF). SEC-UPLC (size exclusion chromatography) and HIC (hydrophobic interaction chromatography) results interpretation for every produced antibody batch.

Région De Lyon, France

Area : Immuno-oncology.Tasks : Development of therapeutics target (membrane receptor) and monoclonal antibodies’ characterisation tests by FRET using flow cytometry. Study of activation markers and IFNg secretion following on NK cells, after NK cells co-culture with antibody candidates.Antibodies screening.Quality control tasks: Quantify and validate antibodies batch produced for functional tests.Scientific skills:Scientific watch.Experiment scheduling, experiments.Results analysis and interpretation.Oral result presentation. (French)Protocol and reports writing.Reagent and lab stock management.Technical skillsCell biology:Cell culture (suspending lines: CHO, adherent, PBMC), transfections, stable cell line elaboration, cells isolation (Ficoll, stemcell method, miltenyi method)Immunology:Flow cytometry (multiparametric, intracellular test, quantitative, FRET), ELISA (binding and inhibition assays).Molecular biology:DNA extraction (after bacteria culture).

Région De Villeurbanne, France

Research Director : Patrick LOMONTEArea: Virology (Herpès Simplex 1) and Genetic.Tasks : Fusion protein construction, following of the protein and viral genome localisation by IF-FISH during the HSV-1 infection. Scientific skills:Scientific watch.Experiment scheduling, experiments.Results analysis and interpretation.Oral result presentation. (French)Protocol and reports writing.Laboratory life engagement.Technical skillsCell biology: Fibroblast cell culture and cell viral infection.Molecular biology: Lentiviral vector cloning, PCR, agarose gel electrophoresis.Microscopy:IF-FISH.

La Baule

Housekeeper and NannyTransverse skills:-take responsibility (child care)-Scheduling and organisationsummer 2014 and 2015

Client advisor and cashierTransverse skills:-scheduling and organisation-good personal relationship skills (customer relationship)-take initiative

Région De Saint-Nazaire, France

Cashier and shelvingTransverse skills:-scheduling and organisation-good personal relationship skills (customer relationship)-take initiative-creativity

Pornichet

Shelving

Master’S Degree In Cell And Molecular Biology , Biotechnology And R&D Speciality

Activities and Societies: host during "la fête de la science"Class realized at l’École Supérieur de Biologie Biochimie (ESTBB), Université.

First Years Of Master’S Degree, Genetic And Cells Biology

Three Years University, Life Science, Cell Biology And Physiology Speciality

Activities and Societies: International exchange in Université du Québec à Chicoutimi, Canada, during the sixth semester.