Responsible for leading and running a small HER-2 team. Plan and organise work flow. Work to strict targets and deadlines in order to achieve set targets in a busy production lab environment.-Growth of Class II HER2 cell lines -Processing HER2 cell cores -Producing paraffin-embedded HER2 cell multi blocks-Cutting HER2 multi blocks using a microtome-Reading the thickness of HER2 cores using an interferometer-Demonstrate a very high level of aseptic technique and environmental monitoring in cell culture-Training of new employees to a very high standardPreviously responsible for running the ELISA department-Measured antibody concentration by Quantitative ELISA-Carried out Total Protein assays using a spectrophotometer-Confirmed antibody isotypes-Prepared antibodies for in-process verification testing-Maintained all ELISA trending databases-Demonstrated a high level of precision pipetting -Calibrated equipment-Planned, organised and maintained ELISA workflow and troubleshooted problems -Maintained stock levels and laboratory equipment.

-Antibody development for use on automated IHC platform-Antibody optimisation -Cutting paraffin-embedded tissue sections using a microtome-Microscopically scoring tissue sections -Writing antibody development summaries, verification reports, in use validation reports, product claims summaries and Device Master Records (DMR’s)-Organising DMR change controls, DMR approvals and SAP updates-Investigation and trouble shooting tissue staining issues relating to customer complaints-Training of new and existing employees -Organising and holding IHC wet-workshops for global customers, distributors and sales account managers-Member of the Health and Safety/Audit team-Member of the Health Advocate team-ROSPA certificated DSE assessor

-Generation of monoclonal antibodies by Somatic Cell Hybridisation-Hybridoma cloning, Hybridoma tissue culture-ELISA-Western Blotting-Immunohistochemistry (bench and automated)-Cutting paraffin-embedded tissue sections using a microtome-Microscopically scoring tissue sections-Maintenance and Calibration of laboratory equipment-Training of new employees-Supervisory duties included providing help and support to a team of Research Associates, co-ordinating and monitoring workloads, monitoring employee absences, holidays, time sheets, general timekeeping and carrying out employee appraisals.

-Generation of monoclonal antibodies by Somatic Cell Hybridisation-Hybridoma cloning, Hybridoma tissue culture-ELISA-Western Blotting-Immunohistochemistry (bench and automated)-Cutting paraffin-embedded tissue sections using a microtome-Microscopically scoring tissue sections-Maintenance and Calibration of laboratory equipment-Training of new employees

-Bench Immunohistochemistry-Cutting paraffin embedded sections using a microtome-Microscopically scoring tissue sections-Cutting fixed tissue sections using a cryostat-Maintaining stock levels and laboratory equipment and preparing laboratory buffers and solutions