Kai Yi (Eldeen) Lim Email & Phone Number

Singapore, Singapore

Working on understanding the pathogenesis of Klebsiella pneumoniae to come up with host-directed therapy!

Singapore

Designed and synthesized a range of antimicrobial peptidomimetics for establishment of the structure-activity relationship against multidrug-resistant gram-positive and -negative bacteria, both alone and in conjunction with antimicrobial peptides respectively.Skills: chemical synthesis, flash column chromatography, bacteria culture, drug testing assays, hemolysis assays, fluorescence microscopy.

Singapore

• Students range from Primary 3 to A-levels.• Math and Science focused

Singapore

Supervisor: Assoc. Prof. Xavier RocaConducted my final year research project titled “Alternative Splicing in Human Myeloid Cell Differentiation and Leukemia” focusing on novel RNA-binding proteins and how their expression levels affect differentiation of myeloid cells.• Prepared and expanded plasmids using HEK293T cells for knocking down or overexpression of target RNA binding proteins.• Induced differentiation of myeloid cells down the different pathways via chemical treatments.• Performed Giemsa staining to capture morphological differences between differentiated cells.• Extracted and reverse transcribed RNA samples for analysis using quantitative PCR (qPCR).• Analyzed cell differentiation in terms of phenotypical differences (surface markers) on treated and transduced myeloid cells using flow cytometry.• Carried out functional assays including netosis, phagocytosis and ROS assays.• Conducted splicing analysis of specific genes.

Singapore

Quantitative Biosciences DepartmentSupervisor: Dr Adrian SimDeveloped and optimized a novel functional-based assay using patient-derived materials for testing of future pipeline projects.Techniques: 3D cell culture; T-cell education and selection; media preparation; cell co-culture; drug testing; Incucyte; Echo Liquid Handler; multiplate readers

Singapore

Supervisors: Assoc. Prof. Andrew Tan Nguan Soon, Dr. Cheng Hong ShengStudied drug resistance in Glioblastoma Multiforme (GBM) using combination therapy by comparing responses of 8 patient-derived pro-neural and mesenchymal cell lines. Project was done in partnership with National Neuroscience Institute (NNI).Techniques: cell culture; cell lysis; liquid-liquid phase separation; HPLC; bradford assay; RNA extraction; reverse transcription; qPCR and data analysis

Singapore

Lymphocyte Signalling Research LaboratorySupervisor: Asst. Prof. Navin Kumar VermaConducted a research project titled “Mechanism of Immunosuppression in the Tumor Microenvironment” which characterized the effects of hypertonic K+ conditions representative of tumor microenvironments on natural killer (NK) cells.Techniques: tissue cell culture; lymphocyte isolation; FACS; high content analysis; cell co-culture experiments; western blotting; cell staining; fluorescence microscopy

Singapore

• Led a team of 17 main committee members and 62 subcommittee members over the year to plan and execute a mix of physical and online events.• Managed 2 separate sub-clubs: Freshmen Orientation Programme (FOP) and Youth Expedition Project (YEP).• Organized the first-ever online School of Biological Sciences (SBS) convocation for over 300 undergraduate, masters and post-graduate students.• Kickstarted an online tutoring program together with School of Humanities (SoH) and School of Physical and Mathematical Sciences (SPMS) by partnering with a series of charity organizations.• Spearheaded the initiation of the Clade (house) system for all SBS students.• Established the Biohackathon sub-club which hosted a total of 6 workshops to educate our students in fields of coding and data analysis.• Chaired the planning of College of Science (CoS) Day 2020 by gathering main committee members across the 3 science schools.

Singapore

• Spearheaded the planning of a total of 4 major School of Biological Sciences events, each for over 400 students.• Explored new event ideas, such as the addition of craft workshop booths to school events. This gathered much positive feedback from both the students and the school as the booths were engaging, yet informative.• Planned the stage programme of College of Science (COS) Day 2019 for over 1000 students and brought interesting and refreshing games to the table. This increased the participation rate from the audience, with the maximum number of participants each game.

Singapore

Microbiology and Systems Biology LaboratorySupervisor: Assoc. Prof. Kevin PetheAssisted with the testing of potential tuberculosis drug combinations on BCG, working off from preliminary hits discovered through drug libraries under Post-Graduate student Lee Bei Shi.Techniques: media preparation; inoculation and culture of bacteria BCG; drug testing

Singapore

(Member Nov'14 - Oct'15; Welfare committee head Oct'15 - Oct'16)• Liaised and planned a week-long overseas service trip with Vietnamese ICs for over 40 volunteers and was responsible for the welfare of Singaporean volunteers while overseas.• Organized local sales to fund raise for purchase of daily necessities and educational materials for donation to shelters and homes in Vietnam.• Initiated the Sugar Ultimate Frisbee Tournament in 2017 with over 100 participants to spread awareness about project cause and raised about $1000 for donation.

Singapore

Supervisor: Prof. Chen Wei Ning, WilliamConducted a research project titled “Investigation and Comparison of Microbial Oil Production of Rhodosporidum Toruloides with use of Differing Diet” involving the investigation of lipid accumulation of Oleaginous yeast to analyze feasibility of using varying diets including waste material to produce biofuel. Techniques: microbial culture techniques; cell lysis; liquid-liquid phase isolation; GC-MS; data analysis

Doctor Of Philosophy - Phd, Immunology And Infectious Disease

Bachelor'S Degree, Biological Sciences And Medicinal Chemistry, First Class Honours

Activities and Societies: Biological Sciences Club • Events Director (AY18/19) • President (AY19/20) • Events Subcommittee (AY20/21) SBS.

Summer School

GMS1000: Duke-NUS Pre-Medical Module • Covered a total of 4 medical research areas (cancer and stem cell biology, metabolic disorders.

Nus High Diploma - 'A' Level Equivalent, Majors In Math, Biology, Chemistry And Physics, Graduated With High Distinction (4.5/5.0)

Activities and societies: AY2017 • Captain | Faraday House (House Champion) • Mentor | Project SUGAR • Core Team, Committee IC | OPT Year.