Directed a small research and development group working in animal health vaccinology (swine, bovine and avian). Built and led the team to meet company goals and customer needs. • Animal challenge model development• Viral and bacterial growth• Chemical inactivation kinetics• Serology, ELISA• Protein science• Statistical analysis (R and Origin Pro)

Spearheaded inactivation kinetics investigations of our antigen products and associated production processes to meet critical internal quality and USDA demands. Assisted with swine research experiments, specifically, experimental design and preparation of pathogenic challenge materials. Primary user of and reference for Trackwise® Quality Management Systems for both the R & D and Diagnostic Departments. Active participant in the training, mentoring, task-management and retention of junior team members. • Recombinant expression of viral capsid proteins in baculovirus and E. coli systems.

• College of Pharmacy Point of Contact for all research laboratory issues• Departmental Laboratory Safety Officer• Managed and coordinated use of all shared laboratory instrumentation, including training, repairs and installation.• Interfaced with instrument field engineers, technical service personnel and UNM Physical Plant Department staff• Used effective written and verbal communications with faculty, staff, researchers, Physical Plant Department staff, and outside technical and sales persons to resolve laboratory operation and infrastructure issues

Research focused on understanding and preventing Staphylococcus aureus pathogenesis and host innate immunity relating to human health• Experimental design, execution and data collection and analysis based on advanced technical approaches including protein characterization using FPLC, flow cytometry, ELISA, and SPR. • Data analysis, interpretation and presentation in both oral and written formats. • Developed and authored study reports, oral presentations and manuscripts for publication.• Organized and maintained laboratory resources (supplies, equipment, inventories) necessary for the smooth day-to-day operations of the laboratory. • Trained and directed staff and student employees to perform required experiments, including training for instrument use, and data analysis/interpretation. Provided explanations of protocols and techniques, as well as the underlying scientific principles at work.

Studied the mechanism and regulation of human Nitric Oxide Synthase as it relates to human health• Protein over-expression and purification. • Preparation of enzyme samples for spectroscopic characterization (including enzyme-ligand complexes, reaction intermediates and various metallocenter redox states).

Researched the function of Ammonia and N-Oxide metabolic enzymes in the nitrogen cycle• Expressed, purified and characterized proteins from the ammonia-oxidizing, chemolithoautotroph bacterium Nitrosomonas europaea proteins, including identifying a new family of cytochrome proteins• Developed a functional Escherichia coli over-expression systems for cytochrome P460, cytochrome c'-beta and nitrosocyanin (native, His-tagged, and cleavable His-tagged)• Successfully over-expressed the membrane-anchored tetraheme cytochrome cm552 using Shewenella oneidensis• Crystallized three proteins from Nitrosomonas europaea (optimization, crystal mounting, model building and refinement)