Technician
Leiden
Research in the area of fragment based drug discovery technologies; with a focus on Protein Production, Purification and Characterisation. The last years my focus shifted towards the field of molecular biology to develop a new, proprietary, in house technique.For my projects I'm involved in planning and set-up of experiments, I execute the experiments, perform the data analysis and data interpretation and I'm involved in design of follow-up experiments.Used laboratory techniques: Transformation, DNA purification, Plasmid purifications, Fragmentation of DNA using various techniques, Cloning, Protein expression in rich media, minimal media and deuterated media, Affinity purification, Size Exclusion Chromatography, Bradford-assays, Thermo Shift Assays, SEC-MALS, Peparation of NMR-samples.