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Scientist with a strong in vivo and in vitro skill set specializing in cellular, immunological, animal studies and surgical procedures. Demonstrated ability to lead and manage multiple projects. Highly motivated and passionate team player with great organizational skills and attention to detail.
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ScientistBeam Therapeutics Jan 2022 - PresentCambridge, Massachusetts, Us -
Principal Research AssociateBeam Therapeutics Dec 2018 - Jan 2022Cambridge, Massachusetts, Us
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Senior Research AssociateCrispr Therapeutics Aug 2016 - Dec 2018Boston, Ma, Us• Responsible for study design and hands-on execution of in vivo rodent studies for LNP formulation optimization and assessment of in vivo gene editing o Formulation QC (ribogreen, endotoxin) o In vivo delivery of formulated gene editing payload via intravenous administration• Responsible for ex vivo/in vitro analysis of mouse, pig and monkey liver samples o Tissue collection & processing for genomic DNA preparation, PCR and Sanger sequencingo RNA and Protein detection in liver tissue lysates (qPCR/ELISA, MSD and CE) o Data analysis, data QC and data reporting. Responsible for generating study report in vitro assays and ex vivo work to validate the animal studies • Responsible for executing hands-on in vitro gene editing studieso LNP QC in mouse and human cell lines: cell culture, transfection and harvest, processing and gene editing analysis via Sanger sequencing for Indels determination -
Senior Research AssociateModerna Therapeutics Mar 2014 - Aug 2016Cambridge, Massachusetts, Us• Responsible for hands-on execution of ex-vivo and in vitro target validation studies for multiple Liver programs:o Developed and performed IHC/IF assays in cells and tissues (frozen and paraffin embedded sections)o In vitro transfection of therapeutic mRNA in hepatic mouse and human cell lineso Assessment of RNA (qPCR) and protein expression (ELISA, MSD) in transfected cellso Assessment of protein cellular localization/co-localization via confocal microscopy -
Research TechnologistMassachusetts General Hospital Sep 2013 - Mar 2014Boston, Ma, UsPerformed lab techniques for ongoing research in diabetes immunobiology and islet transplantation models. • Human and mouse islet cell isolation, culture and transplant in experimental animals• Management of treatments and follow-up of experimental animals• Conducted research in improving islet isolation efficiency and quality• Employed knowledge of human, non-human primate and rodent islet manufacturing process and quality control to educate and train junior level staff -
Senior Research Associate IiiUniversity Of Miami, Miller School Of Medicine, Diabetes Research Institute Feb 2002 - Sep 2013Miami, Fl, UsConducted numerous research tasks at the Preclinical Cell Processing and Translational Models Program at the Diabetes Research Institute (DRI)• Key member of the DRI’s Small Animal Core that provides access to rodent cellular products (islets, bone marrow, MSC), in vivo potency assessment of cellular product, and rodent transplant models to multiple investigators• Performed islet biology, cytoprotection, beta-cell proliferation, islet immunobiology (allo-, xeno-transplant; autoimmunity), immunomodulation and immunological tolerance induction, tissue engineering experiments in vitro and in vivo• Managed, trained and supervised staff and trainees in laboratory and animal techniques -
Research AssociateUniversity Of Miami, Miller School Of Medicine, Diabetes Research Institute Feb 2001 - Feb 2002Miami, Fl, UsPerformed numerous research at the Immunobiology of Islet Transplantation Laboratory, Cell Transplant Center at the Diabetes Research Institute
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InternshipCambridge Hospital Jun 1994 - Jun 1996Internship
Elsie Zahr Akrawi Skills
Elsie Zahr Akrawi Education Details
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Boston UniversityGeneral -
Barry UniversityBiomedical Sciences
Frequently Asked Questions about Elsie Zahr Akrawi
What company does Elsie Zahr Akrawi work for?
Elsie Zahr Akrawi works for Beam Therapeutics
What is Elsie Zahr Akrawi's role at the current company?
Elsie Zahr Akrawi's current role is Scientist.
What is Elsie Zahr Akrawi's email address?
Elsie Zahr Akrawi's email address is ea****@****mtx.com
What is Elsie Zahr Akrawi's direct phone number?
Elsie Zahr Akrawi's direct phone number is (347) 658*****
What schools did Elsie Zahr Akrawi attend?
Elsie Zahr Akrawi attended Boston University, Barry University.
What skills is Elsie Zahr Akrawi known for?
Elsie Zahr Akrawi has skills like Cell Culture, Cell, Elisa, Cell Biology, Immunohistochemistry, Immunology, In Vitro, Flow Cytometry, Pcr, Tissue Culture, Laboratory, Clinical Development.
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