MR-1 Biology Research• xCELLigence TCR-T kill assays, and cell density optimisation assays on numerous primary cancer cells in addition to cancer cell lines.• ELISpot for TCR-T cell activation analysis.• Large-scale T cell transductions using G-Rex systems.• Isolation of T cells from blood and PBMC & routine cell culture.• Flow cytometry (Cytoflex).• Dissemination of experiment results at company R&D meetings.

• Routine mammalian cell culture (hGF, HUVEC, proprietary suspension HEK293, CHO), and cryopreservation.• Molecular cloning of plasmids.• Lentiviral production, purification, and titration by qPCR & TCID50.• Target cell line development using lentiviral transduction.• Design of experiments; immunostaining of specific scFv-generating cells for flow cytometry(Attune NxT), MACS & FACS.• Hitpicking of clonal cells using Solentim Cell Metric Software & Hamilton Robots.• Leading the guide RNA design for target gene knockout.• Primer design and validation using Snapgene software.• CRISPR delivery (lipofection & nucleofection of RNP complexes) in human cancer cell lines (MCF-7, HEK293, Raji, Jurkat, THP-1, U2OS, HeLa, SW480, A549, A431).• Genomic DNA extraction and PCR amplification of target-specific amplicons.• Editing efficiency analysis using sanger sequencing and in-house bioinformatics tools.• Day to day management of technician workloads & training.• SOP documentation drafting.• Dissemination of research at departmental meetings.

Headhunting, Screening and Interviewing of candidates worldwide for job positions based in CyprusRecruitment process managementLiaison with clients (HR, Executive Directors, CEOs) regarding their recruitment needsCommunication with potential candidates regarding their employment & relocation needsOperation of Social Media ChannelsHandling candidate and client enquiriesPreparation and formating of CV'sArranging interviews between clients and candidatesCreating Vacancy contentMaintaining databaseMaintaining website (WordPress)

Part-time job alongside studies

Part-time job alongside studies

Maintaining order and an organised approach whilst running events.Quality communication and marketing skills whilst guiding visitors around the campus.Cooperation with university staff during Registration Week.Temporary, part-time job alongside studies.

Course representative for BSc Biomedical Science cohort.Volunteered and committed time to meet university staff and report issues raised by fellowstudents.Successfully implemented staff training in the use of audio-visual equipment, enhancing studentlearning outside of contact hours.Positive feedback proven by anonymous nominations by fellow students to run as a candidatefor Vice President Voice (Full-time role within the Students’ Union).

Careers and Employability Centre, University of SurreyCalling graduates to explain the purpose of the survey in a clear and helpful manner.Used a positive attitude and negotiation skills to encourage and persuade graduates tocomplete the survey, hence increasing uptake.Temporary, part-time job alongside studies.