Emily Donovan Email and Phone Number
I am a dedicated and detail-oriented engineer with extensive experience in process engineering, upstream process development, and process analytics. My career reflects a commitment to advancing cutting-edge gene therapy platforms, with expertise in upstream process engineering and cross-functional collaboration.At BE Biopharma, I contributed significantly to the development and optimization of novel B cell-based therapies. On the AAV core team, I worked cross functionally with research teams and stakeholders to drive timelines for various vector screenings to support multiple programs. Acting as subject matter expert (SME) for AAV production, I have experience in end-to-end production of viral vectors. My work on the analytical team involved designing and analyzing immunophenotyping panels, developing innovative gating strategies, and streamlining data analysis workflows to support IND enabling studies. I took pride in presenting findings to drive collaboration between teams and ensuring operational efficiency by managing critical lab resources.During my time at CRISPR Therapeutics, I played a pivotal role in advancing AAV production technologies and scaling processes from development to GMP readiness. As an Upstream Engineer, I optimized AAV manufacturing processes using suspension HEK293 cells, executing critical process steps such as vial thaw, triple transient transfection, production bioreactors, and harvest. I acted as the Person-in-Plant (PiP) for the generation of master cell banks, ensuring readiness for GMP-scale operations and collaborating with CMOs for tech transfer.In this role, I leveraged tools like JMP and DoE to design experiments that de-risked cell banks and characterized upstream processes. My work supported multiple campaigns, enabling consistent delivery of high-quality material. Additionally, I conducted flow cytometry analytics, including %CAR assays, to ensure process quality and performance.My ability to translate complex data into actionable insights and author detailed documentation such as SOPs, DOPs, and DBRs further supported the standardization and efficiency of operations. These experiences have enhanced my expertise in bioprocessing and gene therapy development while strengthening my ability to collaborate across cross-functional teams. By working closely with colleagues and partners, I have honed my skills in driving innovation and delivering impactful solutions to address complex challenges in the biopharma space.
Be Biopharma
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Process Engineer Ii Aav | Analytical Development (Through Oct 2024)Be Biopharma Oct 2022 - PresentCambridge, Massachusetts, Us- Executed immunophenotyping panels to enhance understanding of B Cell drug products, generating high quality data with quick turnaround for IND enabling studies.- Designed, analyzed, and reported flow cytometry data across multiple panels, optimizing panels for improved data read-out. Developed experimental plans, interpreted scientific results, and provided statistical analysis.- Developed innovative gating strategies which facilitated diverse data analysis approaches. This included creating standardized analysis templates which improved efficiency of flow cytometry data processing.- Ensured team could perform their roles effectively by managing inventory of reagents and established stock levels.- Presented findings in companywide meetings to disseminate information and foster collaboration across teams. - Delivered high priority AAV constructs and worked with various program leads to understand timelines to consistently deliver high quality material.- Acted as subject matter expert (SME) on end-to-end process, including transfection, harvest, AAVX, IDX, and final formulation. Experienced in upstream and downstream optimization for AAV production. -
Upstream Engineer IiCrispr Therapeutics Feb 2022 - Oct 2022Boston, Ma, Us- Acted as subject matter expert (SME) for AAV upstream process from vial thaw, scale up, triple transient transfection, production bioreactor, and clarification/ harvest.- Represented as person in plant (PiP) by overseeing master cell bank (MCB) generation used for future GMP scale-up AAV runs.- Participated in key meetings and executed multiple characterization campaigns regarding tech transfer of process to various CMOs.- Utilized JMP and DoE in process characterization campaigns as well as designed experiments to de-risk multiple cell banks for future GMP use.- Conducted flow cytometry, running % CAR assays for in-process analytics to support multiple campaigns. -
Upstream Engineer ICrispr Therapeutics Jun 2020 - Feb 2022Boston, Ma, Us- AAV process development role working with 6L-50L single use bioreactors (SUBs). Experienced with triple transient transfection as well as plasmid optimization surrounding transfection- Subject matter expert (SME) on AAV upstream process - Experienced with downstream process operations such as TFF (UF/DF) and Akta purification methods- Authored SOPs, DOP’s, and DBR’s. GMP experience regarding tech transfer from platform development to CMO implementation - Experienced using JMP and DoE -
Incoming Upstream EngineerCrispr Therapeutics Apr 2020 - Jun 2020Boston, Ma, Us -
Upstream Process Development Co-OpBristol-Myers Squibb May 2019 - Jun 2020Lawrence Township, Nj, Us- Accountable for sampling, feeding, and troubleshooting single-use-bioreactors ranging in scale from 15mL to 500L.- Utilized high throughput equipment such as ABMR and TECAN with tasks that included analysis on metabolites, pH readings, and cell viability. bioreactors ranging in size of 15mL to 500L for pH corrections, cell viability and cell counts, as well as metabolites in order to accurately correct feed strategies for CHO cell experiments. -Experience using automated liquid handlers (TECAN), Vicell, and Cedex Bio HT and performing troubleshooting.
Emily Donovan Education Details
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Worcester Polytechnic InstituteBiomedical/Medical Engineering
Frequently Asked Questions about Emily Donovan
What company does Emily Donovan work for?
Emily Donovan works for Be Biopharma
What is Emily Donovan's role at the current company?
Emily Donovan's current role is Process Engineer | Passionate About Process Optimization & AAV Production | Experience With End-To-End Mammalian Cell Culture and Tech Transfer | Cross-Collaboration Enthusiast.
What schools did Emily Donovan attend?
Emily Donovan attended Worcester Polytechnic Institute.
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