Affiliation: Bioprocess Engineering Group - Biomanufacturing and Digital TwinsResearch topics: high-throughput lifestyle and biosafety engineering of microorganisms for industrial cultivation, microbial whole-cell biosensors for biointelligence, and the application of the Safe-and-Sustainable-by-Design framework to bioprocesses and biotechnologies.

Affiliation: Bioprocess Engineering Group - Biomanufacturing and Digital Twins DivisionResearch topics: High-throughput strain engineering and optimisation, bio-intelligence, living biosensors and safe-and-sustianable-by-design biotechnologies.Current projects:- BIOS: The bio-intelligent DBTL cycle, a key enabler catalysing the industrial transformation towards sustainable bio-manufacturing- Embedding Safe-by-Design (SbD) in circular bio-economy workflows: from feedstock to product and valorizable waste- A viral genome recoded with an unnatural base pair- SENSIBAR: Living biosensors for industrial biotechnology- HaloGenie: Unleashing the potential of halophile genomics for enhanced Industrial Biotechnology- BIOSENSEI: Biosensor-based diagnostic platform for real-time monitoring of existing and emerging environmental pollutantsPast projects:- PREP-IBISBA- An exploratory study on implementing Safe-by-Design (SbD) in circular bio-economy researchSupervision and teaching activities:· Current co-promoter of the PhD projects of Alexandra Müller, Sophie van der Vlugt, Güniz Özer-Bergman, Antoine Raynal, Sonia Mecacci and Konstantinos Lampanaris· Bioprocess Engineering BSc and MSc Theses (BPE-80336) Supervisor· iGEM Supervisor (iGEM WUR 2023 PseuPomona)· iGEM Judge 2023· Systems and Synthetic Biology (SSB-50806) Lecturer and Laboratory Instructor· Academic Consultancy Training (ACT) Scientific Advisor

Affiliation: Laboratory of Systems and Synthetic BiologyResearch topics: Biosafety, genetic circuits, gene regulationProjects:- SafeChassis: Implementing and assessing safeguards for lifestyle engineering of a versatile industrial chassis Supervision and teaching activities:· Systems and Synthetic Biology BSc and MSc Theses (SSB-80336) Supervisor· iGEM Supervisor (iGEM WUR 2022 Colourectal)· Introduction to Systems and Synthetic Biology (SSB-50806) Lecturer and Laboratory Instructor

Affiliation: Laboratory of Systems and Synthetic BiologyResearch topics: Biosafety, genetic circuits, genome engineering, metabolic engineering, safe-by-design biotechnologiesProjects:- SafeChassis: Implementing and assessing safeguards for lifestyle engineering of a versatile industrial chassis - EmPowerPutida Supervision and teaching activities:· Systems and Synthetic Biology BSc and MSc Theses (SSB-80336) Supervisor· iGEM Supervisor (iGEM WUR 2019 Xylencer)· Introduction to Systems and Synthetic Biology (SSB-50806) Teaching Assistant · iBiosystems (SSB-20806) Teaching Assistant · Toolbox of Systems and Synthetic Biology (SSB-31312) Instructor

Laboratory of Virology. Reverse genetics system for the very important pathogen Tomato spotted wilt virus (TSWV) by expressing artificial minigenomes in recombinant insect cells.· Cell culture and transfection in mammalian (BSR T7/5) and insect cells (C6/36).· Fluorescence microscopy and cell sorting (FACS).· Molecular biology: PCR, Gibson assembly, site directed mutagenesis, T7 transcription, in vitro translation, RNA extraction, RT PCR, Western blot, immunostaining.

Laboratory of Systems and Synthetic Biology. Microbial Synthetic Biology Group.Studies in synthetic biology, microbiology, genetic and metabolic engineering in fungi. · Bioinformatic analysis, phylogenetic studies: NCBI, Mycocosm JGI, BLAST, Clustal, PROLINE, MEGA7, PFam, PROSITE, SMART.· Cloning and heterologous expression in E. coli and S. cerevisiae: PCR, electrophoresis, Golden Gate, Plasmid isolation, restriction analysis, transformation in bacterial system, transformation in fungal system, Benchling.· Sensitivity assays: culturing, growth tests, fluorescence assays.

Molecular and Parasite Immunology Department, Tropical Diseases Research Centre of the University of Salamanca (CIETUS).Studies in design, production and evaluation of recombinant and chimeric proteins in bacterial systems as potential vaccines against Fasciola hepatica.- Design: EntelechonGmbH, Kazusa, GenScrip, bacterial culture, bacterial transformation, PCR, plasmid isolation, restriction digestion.- Production: protein production, protein purification, protein electrophoresis, Western Blotting, dialysis, freeze drying.- Evaluation: ELISA, Derek Nexus, citotoxicity, in vitro macrophages culture.

Adaptive Mechanisms for Plant Biotechnology and Energy Crops Research Group, Institute ofMolecular and Cellular Biology of Plants.Research in Euphorbia lathyris and laticifers cell as potential energy system for the production of molecules with high added value in the fields of renewable energy, and second and third generation biofuels. · Analysis of ecotypes· Studies of tetraploidy· Plant transformation: A. tumefaciens, biolistic, GUS assays· Fungi infection: microscopy· Latic cells· Bioinformatics: metabolomics