Enrico Porotti Email & Phone Number

Straubing, Bavaria, Germany

Aalborg, North Denmark Region, Denmark

Overexpression and secretion of PET-hydrolyzing enzymes in Pseudomonas putida KT2440.Tasks:- Design of plasmids, primers, pipelines for assembly of plasmids;- Kit-based DNA purification, touch-down PCR, restriction-and-ligation cloning;- Agarose gel electrophoresis of DNA and imaging of gels;- Bacteria transformation by electroporation;- Selection and verification of successful transformants;- Lab-scale protein expression experiments;- SDS-PAGE for analysis… Show more Overexpression and secretion of PET-hydrolyzing enzymes in Pseudomonas putida KT2440.Tasks:- Design of plasmids, primers, pipelines for assembly of plasmids;- Kit-based DNA purification, touch-down PCR, restriction-and-ligation cloning;- Agarose gel electrophoresis of DNA and imaging of gels;- Bacteria transformation by electroporation;- Selection and verification of successful transformants;- Lab-scale protein expression experiments;- SDS-PAGE for analysis of intra- and extracellular proteins;- Target protein purification (IMAC with ÄKTA Start, diafiltration);- Protein assays (Bradford, esterase activity, PET hydrolysis);- Assorted troubleshooting of issues with instruments and processes.Results:- Assembled an expression vector for a protein of interest based on a SEVA plasmid;- Demonstrated expression at low concentrations of an engineered PET-hydrolyzing enzyme in P. putida, with quality of the protein comparable to that obtainable with an E. coli expression system;- Identified issues with the adopted approach for heterologous protein secretion engineering in P. putida and suggested alternatives from the literature. Show less

Lisbon, Portugal

Early stage researcher in the MSCA-ITN FertiCycle, project on hygienisation of pig slurry by pH modification.

Austria

Development, validation and uncertainty balance of an ICP-OES method for the quantification of plant available and amorphous silicon in arable land and grassland soils of Austria.Investigated:- Effect of instrument settings on RSD of measurements at different analyte concentrations, linearity of calibration curves, LOD and LOQ;- Existence of a carry-over effect and influence of rinse time;- Existence of a matrix effect and influence of sample dilution, soil chemical… Show more Development, validation and uncertainty balance of an ICP-OES method for the quantification of plant available and amorphous silicon in arable land and grassland soils of Austria.Investigated:- Effect of instrument settings on RSD of measurements at different analyte concentrations, linearity of calibration curves, LOD and LOQ;- Existence of a carry-over effect and influence of rinse time;- Existence of a matrix effect and influence of sample dilution, soil chemical characteristics and instrument settings;- Analyte recovery;- Effect of solid-liquid separation technique and shaking speed on estimated analyte concentrations;- Uncertainty balances of estimated analyte concentrations using Kragten's approach and distribution of uncertainty across various uncertainty sources.All data analysis performed with R using custom-written functions for instrument calibration and uncertainty balances and FAMD (Factor Analysis for Mixed Data). Show less

Copenhagen, Danimarca

Preliminary trials on Greenlandic glacial rock flour (GRF), as basis for a PhD research project:- Physical (water retention) and chemical (pH, P availability) analyses on soils treated with GRF;- Nitrogen fixation rate estimate of plants grown on soils treated with GRF;- Chemical characterization of GRF by means of a sequential extraction procedure and total digestion and quantification with ICP-OES;- Fractionation of GRF based on particle size;- Set up of a pot trial with… Show more Preliminary trials on Greenlandic glacial rock flour (GRF), as basis for a PhD research project:- Physical (water retention) and chemical (pH, P availability) analyses on soils treated with GRF;- Nitrogen fixation rate estimate of plants grown on soils treated with GRF;- Chemical characterization of GRF by means of a sequential extraction procedure and total digestion and quantification with ICP-OES;- Fractionation of GRF based on particle size;- Set up of a pot trial with maize and different Danish soils treated with GRF of different origin. Show less

Milan Area, Italy

Performance of a pot trial on lettuce with sewage sludge as N fertiliser, and cooperation in routine in-house analyses on wastewater and treated water.

Master'S Degree, Msc Engineering (Sustainable Biotechnology)

Master's program focused on the conversion of biomass and biorefineries: - Biorefinery principles; - Systems and synthetic biology.

Master'S Degree, Msc Engineering (Sustainable Biotechnology)

Exchange student, as continuation of my master's degree from AAU. - Design of experiments and optimisation - Yeast physiology.

Master’S Degree, Environmental Science, Gpa 3.6/4.0

Second year of EnvEuro double degree master's programme, specialization "Soil Resources and Land Use". Consolidation and expansion.

Master’S Degree, Agriculture; Specialisation: Environmental Sciences – Soil, Water And Biodiversity

First year of the EnvEuro double degree master's programme, specialization "Soil science and land use". During this year I mainly focused.

Laurea Triennale, Scienze E Tecnologie Agrarie, 103/110

Three years programme on agricultural sciences and technologies (agronomy, crop science, arboriculture, animal productions, agricultural.