• Cultured parental and radiation resistant human prostate cancer cell lines• Transfected cells with siRNA and assessed phenotype change using clonogenic assay, proliferation assay, and invasion assay• Extracted DNA, miRNA, serum, and plasma from cancer patient blood and urine samples• Assessed lactate production and mitochondrial respiration in parental and radiation-resistance cells under normoxia and hypoxia• Generated cDNA from serum exosomal RNA and measured lncRNA levels… Show more • Cultured parental and radiation resistant human prostate cancer cell lines• Transfected cells with siRNA and assessed phenotype change using clonogenic assay, proliferation assay, and invasion assay• Extracted DNA, miRNA, serum, and plasma from cancer patient blood and urine samples• Assessed lactate production and mitochondrial respiration in parental and radiation-resistance cells under normoxia and hypoxia• Generated cDNA from serum exosomal RNA and measured lncRNA levels using qPCR• Optimized and performed a protocol for detection of aSMase activity in cancer patient serum Show less

• Prepared growth media and cultured Halobacteria• Extracted and purified nano-scaled gas vesicles from cultured Halobacteria• Assessed concentrations of gas vesicles using Nanodrop and compiled measurements in database• Designed and optimized tissue-mimicking agar phantoms to test novel ultrasound contrast agents• Imaged gas vesicles in gel phantoms under ultrasound using VisualSonics Vevo2100 System• Conducted literature searches to generate Kupffer cell… Show more • Prepared growth media and cultured Halobacteria• Extracted and purified nano-scaled gas vesicles from cultured Halobacteria• Assessed concentrations of gas vesicles using Nanodrop and compiled measurements in database• Designed and optimized tissue-mimicking agar phantoms to test novel ultrasound contrast agents• Imaged gas vesicles in gel phantoms under ultrasound using VisualSonics Vevo2100 System• Conducted literature searches to generate Kupffer cell immunohistochemistry staining protocol and optimized experimental parameters• Performed immunofluorescence staining of mouse liver tissues and imaged sections using confocal fluorescence microscopy Show less

• Conducted polymerase chain reactions for detection of pathogen resistant genes in common bean populations• Screened DNA genotype for bacterial pathogen resistance using QIAxcelScreenGel program and agarose gel electrophoresis• Maintained and updated database of bean parents and populations information • Cultured populations of anthracnose and common bean blight bacteria and inoculated cultured bacteria to bean populations• Performed DNA extraction with frozen and fresh leaf… Show more • Conducted polymerase chain reactions for detection of pathogen resistant genes in common bean populations• Screened DNA genotype for bacterial pathogen resistance using QIAxcelScreenGel program and agarose gel electrophoresis• Maintained and updated database of bean parents and populations information • Cultured populations of anthracnose and common bean blight bacteria and inoculated cultured bacteria to bean populations• Performed DNA extraction with frozen and fresh leaf samples Show less