Erik Pendleton Email & Phone Number

Greater San Diego Area

San Diego, Ca

San Diego, Ca

Investigated biological effects of small-molecule inhibitors of the MAPK/ERK pathway. Developed Gene Expression profiling in support of translational efforts. Investigated lysosomal drug sequestation.

Leading cellular mechanism-of-action studies for a small-molecule program targeting cancer metabolism. Developed and validated high-content imaging assays to determine cytological effects of inhibitors of stress metabolism pathways. Generating proof-of-concept studies demonstrating sensitivity of leukemia cells to pharmacological alterations of epigenetic and metabolic pathways.Managing compound progression and reporting to project team.

Greater San Diego Area

Working on advanced biotechnology R&D projects. • Worked on development of a portable system capable of manufacturing therapeutic proteins in the field (patent pending): Sullivan, C.J., Pendleton, E.D., Sasmor, H.H., Hicks, W.L., Farnum, J.B., Muto, M., Amendt, E.M., Schoborg, J.A., Martin, R.W., Clark, L.G., Anderson, M.J., Choudhury, A., Fior, R., Lo,Y.H.,Griffey, R.H., Chappell, S.A., Jewett, M.C., Mauro, V.P., and Dresios, J. 2015. A cell-free expression and purification process for rapid production of protein biologics. Submitted.- Developed novel cell-free protein expression systems for expression of therapeutic proteins- Methods optimization for protein purification from complex cellular mixtures- Analyzed biological activity of manufactured proteins• Developed novel method of identifying genetically modified organisms (GMOs):Challise J.Sullivan 1, Erik D.Pendleton 1, Rachel E.Abrams 1, David L.Valente, Michelle L.Alvarez, Richard H.Griffey, John Dresios "Chromatin structure analysis enables detection of DNAinsertions into the mammalian nuclear genome' Biochemistry and Biophysics Reports 2(2015)143–152- Researched, set up and performed ChIP-sequencing to characterize epigenetic signatures of GMOs- Designed probes for interrogating methylation changes in GMOs• Developed system to stop bleeding of internal wounds (patent #8,663,932)- Identified wound-specific markers- Designed and fabricated wound-binding microspheres; demonstrated specific microparticle binding to injured sites in animal models of lethal injury• Developed biosensors capable of detecting explosive materials- Protein engineered >100 GPCRs (membrane odor receptors)- Developed in vitro system to express membrane receptor proteins- Developed assays to detect bioactivity of odor receptors in vitro

Greater San Diego Area

Investigated pharmacodynamic/pharmacokinetic properties of Hemoglobin Based Oxygen Carriers (HBOCs)• Developed in vivo models to investigate physiological effects of HBOCs on transgenic mice expressing the human sickle cell mutation.- Anesthetized mice, set up mechanical ventilation, monitored blood gas and blood pressure.- Induced sickle state and monitored blood flow changes with a laser doppler. • Investigated pharmacokinetics of HBOCs in a rat model.- Helped develop ELISA assay used to quantify Polyethylene Glycol (PEG) and PEG-coated molecules at nanomolar levels in tissue and serum.- Surgically installed cannulas in rats, administered HBOCs and monitored drug metabolism.

Greater San Diego Area

- Helped improve in vitro oncogene discovery system using ribozyme/siRNA libraries to screen human cell lines for genes involved with ligand induced apoptosis. - Created stable and transiently transfected cell lines- Performed sub-cloning/plasmid construction. - Optimized ribozyme/siRNA library extraction from transduced cell cultures. - Utilized TUNEL assays/FACS to isolate apoptotic cells.

Greater San Diego Area

Tested sensitive HIV-1/HCV blood screening assay for FDA approval

Master Of Science (M.S.), Biological Sciences, 3.59

Activities and Societies: Taught laboratory courses as a Teacher's Assistant - Investigated mechanisms controlling neuronal growth.

Bachelor Of Science (Bs), Biochemistry, 3.52

Activities and Societies: Alpha Epsilon Delta As a Research Student: - Investigated ecto-ATPase activity. - Co-authored ‘Regulation.