Erik Soons Email & Phone Number

Leuven, Vlaanderen, België

Gent, Vlaanderen, België

- Screening technologies assay development: GPCR modulating compounds & nanobodies-Small molecule screening (binding affinity) on target GPCRs through competitive radioligand displacement-Transient expression of target GPCRs , membrane preparations for screenings.

Mechelen

High throughput screening of compound libraries in in vitro and cellular assays to identify hits and to support hit-to-lead optimization in drug discovery projects. Experience in: -Biochemical/cellular assay development-Screening of small-molecule drug candidates-Mammalian cell culture-Liquid handling robotics

Bimolecular fluorescence complementation in Candida albicans – an in vivo tool for protein-protein interaction detection Duties: Design and implementation of an optimized split-GFP assay in the fungus C. albicans. Usage of recombinant DNA-technology to create C. albicans strains expressing target proteins fused to split-GFP protein fragments for interaction analysis by fluorescence laser-scanning microscopy.

Ku Leuven

Confocal microscopic analysis of retinal ganglion cell degeneration in N-methyl-D-aspartate -injected mice (glaucoma model).Duties: Immunohistochemical staining of Brn3a in whole mount mouse retinas. Confocal fluorescence imaging of stained retinal ganglion cells. Computational analysis of cell degeneration via AxioVision.-Introducing an overexpression mutation in a lac repressor promoter to abolish leaky expression of a mutant phi29 DNA polymerase/g3p fusion protein on a phage… Show more Confocal microscopic analysis of retinal ganglion cell degeneration in N-methyl-D-aspartate -injected mice (glaucoma model).Duties: Immunohistochemical staining of Brn3a in whole mount mouse retinas. Confocal fluorescence imaging of stained retinal ganglion cells. Computational analysis of cell degeneration via AxioVision.-Introducing an overexpression mutation in a lac repressor promoter to abolish leaky expression of a mutant phi29 DNA polymerase/g3p fusion protein on a phage display vectorDuties: Apply PCR-directed mutagenesis to incorporate a mutation in a promoter element. Directional cloning of the mutated element into a display vector, followed by sequencing of the construct. Transformation of the construct into E. coli host and evaluation of its growth. Show less

-One month internship in the laboratory for animal physiology & signal transduction:Purification & functional characterisation of proteolytic enzymes of plague insects-Two month internship in the laboratory for fluorescence microscopy & spectroscopy:Deducing the mechanism of In vivo DNA scanning by the human transcription co-activator LEDGF/p75

Bachelor - Master, Biochemistry & Biotechnology, Cum Laude

Research orientation: - Plant & fungal cell biology

3De Graad Aso Wiskunde (6U.) - Wetenschappen, Mathematics And Science